Cortactin (p80/85) Antibody, clone 4F11 | EMD Millipore 05-180 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cortactin (p80/85) Antibody, clone 4F11

catalog :

05-180

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4F11

The same clone is also sold as:

Abcam: ab33333

reactivity :

African green monkey, human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 60

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2a	Bertier L, Boucherie C, Zwaenepoel O, Vanloo B, Van Troys M, Van Audenhove I, et al. Inhibitory cortactin nanobodies delineate the role of NTA- and SH3-domain-specific functions during invadopodium formation and cancer cell invasion. FASEB J. 2017;31:2460-2476 pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig s4b	Gopal S, Veracini L, Grall D, Butori C, Schaub S, Audebert S, et al. Fibronectin-guided migration of carcinoma collectives. Nat Commun. 2017;8:14105 pubmed publisher
immunocytochemistry; human; loading ...; fig 4d	Bichet M, Touquet B, Gonzalez V, Florent I, Meissner M, Tardieux I. Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces. BMC Biol. 2016;14:97 pubmed
immunocytochemistry; human; loading ...; fig 1c	Zhang Y, Nolan M, Yamada H, Watanabe M, Nasu Y, Takei K, et al. Dynamin2 GTPase contributes to invadopodia formation in invasive bladder cancer cells. Biochem Biophys Res Commun. 2016;480:409-414 pubmed publisher
immunocytochemistry; human; 1:100; fig 7	Qi L, Jafari N, Li X, Chen Z, Li L, Hytönen V, et al. Talin2-mediated traction force drives matrix degradation and cell invasion. J Cell Sci. 2016;129:3661-3674 pubmed
immunocytochemistry; human; loading ...; fig 3e western blot; human; loading ...; fig 4a	BinamÃ© F, Bidaud Meynard A, Magnan L, Piquet L, Montibus B, Chabadel A, et al. Cancer-associated mutations in the protrusion-targeting region of p190RhoGAP impact tumor cell migration. J Cell Biol. 2016;214:859-73 pubmed publisher
immunocytochemistry; human; fig 2 western blot; human; fig 4	Yamada H, Takeda T, Michiue H, Abe T, Takei K. Actin bundling by dynamin 2 and cortactin is implicated in cell migration by stabilizing filopodia in human non-small cell lung carcinoma cells. Int J Oncol. 2016;49:877-86 pubmed publisher
immunocytochemistry; human; loading ...; fig 4a western blot; human; fig 5b	Sinha S, Hoshino D, Hong N, Kirkbride K, Grega Larson N, Seiki M, et al. Cortactin promotes exosome secretion by controlling branched actin dynamics. J Cell Biol. 2016;214:197-213 pubmed publisher
immunocytochemistry; human; fig 2	Duran C, Lee D, Jung J, Ravi S, Pogue C, Toussaint L, et al. NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-?B pathway. Oncogenesis. 2016;5:e231 pubmed publisher
immunocytochemistry; human; fig 1d	Daubon T, Spuul P, Alonso F, Fremaux I, Genot E. VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. J Cell Sci. 2016;129:2586-98 pubmed publisher
immunocytochemistry; rat; 1:100; fig 5	Chojnacka K, Bilinska B, Mruk D. Interleukin 1alpha-induced disruption of the Sertoli cell cytoskeleton affects gap junctional communication. Cell Signal. 2016;28:469-480 pubmed publisher
immunocytochemistry; human; 1:75; loading ...; fig 2I	Lauzier A, Lavoie R, Charbonneau M, Gouin Boisvert B, Harper K, Dubois C. Snail Is a Critical Mediator of Invadosome Formation and Joint Degradation in Arthritis. Am J Pathol. 2016;186:359-74 pubmed publisher
immunocytochemistry; human; fig 1	Yu D, Makkar G, Strickland D, Blanpied T, Stumpo D, Blackshear P, et al. Myristoylated Alanine-Rich Protein Kinase Substrate (MARCKS) Regulates Small GTPase Rac1 and Cdc42 Activity and Is a Critical Mediator of Vascular Smooth Muscle Cell Migration in Intimal Hyperplasia Formation. J Am Heart Assoc. 2015;4:e002255 pubmed publisher
immunocytochemistry; human; fig 2 western blot; human; fig 3	Hong N, Qi A, Weaver A. PI(3,5)P2 controls endosomal branched actin dynamics by regulating cortactin-actin interactions. J Cell Biol. 2015;210:753-69 pubmed publisher
western blot; rat; 1:2000	Olson M, Ingebretson A, Harmelink K. Hippocampal Cortactin Levels are Reduced Following Spatial Working Memory Formation, an Effect Blocked by Chronic Calpain Inhibition. Brain Sci. 2015;5:241-57 pubmed publisher
immunoprecipitation; human western blot; human	Hammer A, Laghate S, Diakonova M. Src tyrosyl phosphorylates cortactin in response to prolactin. Biochem Biophys Res Commun. 2015;463:644-9 pubmed publisher
western blot; human; fig s3	Cao H, Eppinga R, Razidlo G, Krueger E, Chen J, Qiang L, et al. Stromal fibroblasts facilitate cancer cell invasion by a novel invadopodia-independent matrix degradation process. Oncogene. 2016;35:1099-1110 pubmed publisher
immunocytochemistry; human	Gryaznova T, Kropyvko S, Burdyniuk M, Gubar O, Kryklyva V, Tsyba L, et al. Intersectin adaptor proteins are associated with actin-regulating protein WIP in invadopodia. Cell Signal. 2015;27:1499-508 pubmed publisher
immunocytochemistry; human; 1:100 western blot; human	Spring K, Fournier P, Lapointe L, Chabot C, Roussy J, Pommey S, et al. The protein tyrosine phosphatase DEP-1/PTPRJ promotes breast cancer cell invasion and metastasis. Oncogene. 2015;34:5536-47 pubmed publisher
immunocytochemistry; human; 1:100; fig 7	Kim S, Wen W, Prowse P, Hamilton D. Regulation of matrix remodelling phenotype in gingival fibroblasts by substratum topography. J Cell Mol Med. 2015;19:1183-96 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:200	Gendronneau G, Sanii S, Dang T, Deshayes F, Delacour D, Pichard E, et al. Overexpression of galectin-7 in mouse epidermis leads to loss of cell junctions and defective skin repair. PLoS ONE. 2015;10:e0119031 pubmed publisher
immunohistochemistry; zebrafish ; 1:100	Lam P, Mangos S, Green J, Reiser J, Huttenlocher A. In vivo imaging and characterization of actin microridges. PLoS ONE. 2015;10:e0115639 pubmed publisher
immunocytochemistry; African green monkey; fig s3a	Boucrot E, Ferreira A, Almeida Souza L, Debard S, Vallis Y, Howard G, et al. Endophilin marks and controls a clathrin-independent endocytic pathway. Nature. 2015;517:460-5 pubmed publisher
immunocytochemistry; human; fig s1	Sun J, Lu F, He H, Shen J, Messina J, Mathew R, et al. STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion. J Cell Biol. 2014;207:535-48 pubmed publisher
immunocytochemistry; human	GarcÃa E, Machesky L, Jones G, AntÃ³n I. WIP is necessary for matrix invasion by breast cancer cells. Eur J Cell Biol. 2014;93:413-23 pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig 1a	Majeed S, Vasudevan L, Chen C, Luo Y, Torres J, Evans T, et al. Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration. Nat Commun. 2014;5:3891 pubmed publisher
immunocytochemistry; mouse	Lysko D, Putt M, Golden J. SDF1 reduces interneuron leading process branching through dual regulation of actin and microtubules. J Neurosci. 2014;34:4941-62 pubmed publisher
western blot; human	Truffi M, Dubreuil V, Liang X, Vacaresse N, Nigon F, Han S, et al. RPTP? controls epithelial adherens junctions, linking E-cadherin engagement to c-Src-mediated phosphorylation of cortactin. J Cell Sci. 2014;127:2420-32 pubmed publisher
immunocytochemistry; human; 10 ug/ml; fig 2	Georgess D, Mazzorana M, Terrado J, Delprat C, Chamot C, Guasch R, et al. Comparative transcriptomics reveals RhoE as a novel regulator of actin dynamics in bone-resorbing osteoclasts. Mol Biol Cell. 2014;25:380-96 pubmed publisher
immunocytochemistry; human	Sun J, He H, Pillai S, Xiong Y, Challa S, Xu L, et al. GATA3 transcription factor abrogates Smad4 transcription factor-mediated fascin overexpression, invadopodium formation, and breast cancer cell invasion. J Biol Chem. 2013;288:36971-82 pubmed publisher
immunocytochemistry; human; 1:200	Burger K, Learman B, Boucherle A, Sirintrapun S, Isom S, Diaz B, et al. Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cells. Prostate. 2014;74:134-48 pubmed publisher
western blot; human	Thapa N, Choi S, Hedman A, Tan X, Anderson R. Phosphatidylinositol phosphate 5-kinase I?i2 in association with Src controls anchorage-independent growth of tumor cells. J Biol Chem. 2013;288:34707-18 pubmed publisher
immunocytochemistry; human	Gal J, Chen J, Barnett K, Yang L, Brumley E, Zhu H. HDAC6 regulates mutant SOD1 aggregation through two SMIR motifs and tubulin acetylation. J Biol Chem. 2013;288:15035-45 pubmed publisher
immunoprecipitation; mouse western blot; mouse	Yamada H, Abe T, Satoh A, Okazaki N, Tago S, Kobayashi K, et al. Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia. J Neurosci. 2013;33:4514-26 pubmed publisher
immunocytochemistry; rat immunocytochemistry; human immunocytochemistry; mouse	Juin A, Planus E, Guillemot F, Horáková P, Albiges Rizo C, Genot E, et al. Extracellular matrix rigidity controls podosome induction in microvascular endothelial cells. Biol Cell. 2013;105:46-57 pubmed publisher
immunocytochemistry; human; 1:1000 western blot; human; 1:1000	Eckert M, Lwin T, Chang A, Kim J, Danis E, Ohno Machado L, et al. Twist1-induced invadopodia formation promotes tumor metastasis. Cancer Cell. 2011;19:372-86 pubmed publisher
	Sakamoto K, Ozaki T, Ko Y, Tsai C, Gong Y, Morozumi M, et al. Glycan sulfation patterns define autophagy flux at axon tip via PTPRσ-cortactin axis. Nat Chem Biol. 2019;15:699-709 pubmed publisher
	Wang R, Tan J, Chen T, Han H, Tian R, Tan Y, et al. ATP13A2 facilitates HDAC6 recruitment to lysosome to promote autophagosome-lysosome fusion. J Cell Biol. 2019;218:267-284 pubmed publisher
	Fort L, Batista J, Thomason P, Spence H, Whitelaw J, Tweedy L, et al. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol. 2018;20:1159-1171 pubmed publisher
	Morris D, Popp J, Tang L, Gibbs H, Schmitt E, Chaki S, et al. Nck deficiency is associated with delayed breast carcinoma progression and reduced metastasis. Mol Biol Cell. 2017;28:3500-3516 pubmed publisher
	Khan M, Salomaa S, Jacquemet G, Butt U, Miihkinen M, Deguchi T, et al. The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex. J Cell Sci. 2017;130:3094-3107 pubmed publisher
	Schaefer A, van Duijn T, Majolee J, Burridge K, Hordijk P. Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro. J Immunol. 2017;198:4823-4836 pubmed publisher
	Moniz L, Surinova S, Ghazaly E, Velasco L, Haider S, Rodríguez Prados J, et al. Phosphoproteomic comparison of Pik3ca and Pten signalling identifies the nucleotidase NT5C as a novel AKT substrate. Sci Rep. 2017;7:39985 pubmed publisher
	Buck K, Schaefer A, Schoonderwoert V, Creamer M, Dufresne E, Forscher P. Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation. Mol Biol Cell. 2017;28:98-110 pubmed publisher
	Curado F, Spuul P, EgaÃ±a I, Rottiers P, Daubon T, Veillat V, et al. ALK5 and ALK1 play antagonistic roles in transforming growth factor Î²-induced podosome formation in aortic endothelial cells. Mol Cell Biol. 2014;34:4389-403 pubmed publisher
	Payne L, Eves R, Jia L, Mak A. p53 Down regulates PDGF-induced formation of circular dorsal ruffles in rat aortic smooth muscle cells. PLoS ONE. 2014;9:e108257 pubmed publisher
	Grass G, Tolliver L, Bratoeva M, Toole B. CD147, CD44, and the epidermal growth factor receptor (EGFR) signaling pathway cooperate to regulate breast epithelial cell invasiveness. J Biol Chem. 2013;288:26089-104 pubmed publisher
	Shimamura S, Sasaki K, Tanaka M. The Src substrate SKAP2 regulates actin assembly by interacting with WAVE2 and cortactin proteins. J Biol Chem. 2013;288:1171-83 pubmed publisher
	Meiler E, Nieto Pelegrin E, Martinez Quiles N. Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading. PLoS ONE. 2012;7:e33662 pubmed publisher
	Magalhaes M, Larson D, Mader C, Bravo Cordero J, Gil Henn H, Oser M, et al. Cortactin phosphorylation regulates cell invasion through a pH-dependent pathway. J Cell Biol. 2011;195:903-20 pubmed publisher
	Burger K, Davis A, Isom S, Mishra N, Seals D. The podosome marker protein Tks5 regulates macrophage invasive behavior. Cytoskeleton (Hoboken). 2011;68:694-711 pubmed publisher
	Vega F, Fruhwirth G, Ng T, Ridley A. RhoA and RhoC have distinct roles in migration and invasion by acting through different targets. J Cell Biol. 2011;193:655-65 pubmed publisher
	Baumgartner M. Theileria annulata promotes Src kinase-dependent host cell polarization by manipulating actin dynamics in podosomes and lamellipodia. Cell Microbiol. 2011;13:538-53 pubmed publisher
	Bañón Rodríguez I, Monypenny J, Ragazzini C, Franco A, Calle Y, Jones G, et al. The cortactin-binding domain of WIP is essential for podosome formation and extracellular matrix degradation by murine dendritic cells. Eur J Cell Biol. 2011;90:213-23 pubmed publisher
	Tahirovic S, Hellal F, Neukirchen D, Hindges R, Garvalov B, FLYNN K, et al. Rac1 regulates neuronal polarization through the WAVE complex. J Neurosci. 2010;30:6930-43 pubmed publisher
	Liang Y, Niederstrasser H, Edwards M, Jackson C, Cooper J. Distinct roles for CARMIL isoforms in cell migration. Mol Biol Cell. 2009;20:5290-305 pubmed publisher
	Sun X, Li C, Zhuang C, Gilmore W, Cobos E, Tao Y, et al. Abl interactor 1 regulates Src-Id1-matrix metalloproteinase 9 axis and is required for invadopodia formation, extracellular matrix degradation and tumor growth of human breast cancer cells. Carcinogenesis. 2009;30:2109-16 pubmed publisher
	Gendronneau G, Sidhu S, Delacour D, Dang T, Calonne C, Houzelstein D, et al. Galectin-7 in the control of epidermal homeostasis after injury. Mol Biol Cell. 2008;19:5541-9 pubmed publisher
	Shin H, Hayashi M, Christoforidis S, Lacas Gervais S, Hoepfner S, Wenk M, et al. An enzymatic cascade of Rab5 effectors regulates phosphoinositide turnover in the endocytic pathway. J Cell Biol. 2005;170:607-18 pubmed
	Svitkina T, Bulanova E, Chaga O, Vignjevic D, Kojima S, Vasiliev J, et al. Mechanism of filopodia initiation by reorganization of a dendritic network. J Cell Biol. 2003;160:409-21 pubmed

product information

Catalog Number :

05-180

Subcategory :

Cell Structure

Product Name :

Anti-Cortactin (p80/85) Antibody, clone 4F11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q14247

Host Name :

Mouse

Antigen :

Cortactin (p80/85)

Clone :

4F11

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Cortactin (p80/85) Antibody, clone 4F11

Cross Reactivity :

Avian;Bovine;Human;Hamster;Mouse;Rat

Background :

Cortactin is present as a p80 and p85 kDa protein in many cell types. The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding. This repeat region occupies about a third of the protein. The repeat region is followed by a helical region and then a proline rich region and finally at the C-terminus, an SH3 domain. The proline rich domain is thought to bind the SH2 domain of v-src the viral version of the normal c-src known to phosphorylate cortactin. Cortactin bundles microfilaments and this bundling is inhibited by tyrosine phosphorylation. One pathway that activates the pathway leading to tyrosine phosphorylation of cortactin results from a cell adhesion molecule in brain micro-vessel endothelial cells, another is the growth factor, but presumably the very many mechanisms for pp60src activation would also result in cortactin phosphorylation and subsequent loss of actin binding.

Immunogen :

Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src

Specificity :

Recognizes Cortactin.

Package Size :

200 μg

Uses :

Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.