Calmodulin Antibody | EMD Millipore 05-173 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Calmodulin Antibody

catalog :

05-173

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

Japanese quail, shrews, human, mouse, rat, fruit fly , Xenopus laevis

application :

western blot, immunocytochemistry, immunoprecipitation, radioimmunoassay, immunohistochemistry - frozen section, proximity ligation assay

citations: 31

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 2c	Liu G, Papa A, Katchman A, Zakharov S, Roybal D, Hennessey J, et al. Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics. Nature. 2020;577:695-700 pubmed publisher
western blot; human; loading ...; fig 8a	Søndergaard M, Liu Y, Larsen K, Nani A, Tian X, Holt C, et al. The Arrhythmogenic Calmodulin p.Phe142Leu Mutation Impairs C-domain Ca2+ Binding but Not Calmodulin-dependent Inhibition of the Cardiac Ryanodine Receptor. J Biol Chem. 2017;292:1385-1395 pubmed publisher
western blot; Japanese quail; loading ...; fig 6a	Raffeiner P, Schraffl A, Schwarz T, RÃ¶ck R, Ledolter K, Hartl M, et al. Calcium-dependent binding of Myc to calmodulin. Oncotarget. 2017;8:3327-3343 pubmed publisher
western blot; rat; 1:5	Farley M, Swulius M, Waxham M. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. Neuroscience. 2015;304:286-301 pubmed publisher
proximity ligation assay; rat; 1:500; loading ...; fig 3g immunohistochemistry - frozen section; rat; 1:500; loading ...; fig 3b	Caminos E, Vaquero C, Martinez Galan J. Relationship between rat retinal degeneration and potassium channel KCNQ5 expression. Exp Eye Res. 2015;131:1-11 pubmed publisher
western blot; shrews; 1:1000	Zhong W, Hutchinson T, Chebolu S, Darmani N. Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva). PLoS ONE. 2014;9:e104718 pubmed publisher
immunocytochemistry; fruit fly ; 1:500; fig s1	Galletta B, Guillen R, Fagerstrom C, Brownlee C, Lerit D, Megraw T, et al. Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies. Mol Biol Cell. 2014;25:2682-94 pubmed publisher
western blot; mouse; 1:500	Li R, Tan J, Chen L, Feng J, Liang W, Guo X, et al. Iqcg is essential for sperm flagellum formation in mice. PLoS ONE. 2014;9:e98053 pubmed publisher
western blot; human	Newman A, Selkoe D, Dettmer U. A new method for quantitative immunoblotting of endogenous ?-synuclein. PLoS ONE. 2013;8:e81314 pubmed publisher
western blot; rat	Belzil C, Neumayer G, Vassilev A, Yap K, Konishi H, Rivest S, et al. A Ca2+-dependent mechanism of neuronal survival mediated by the microtubule-associated protein p600. J Biol Chem. 2013;288:24452-64 pubmed publisher
western blot; human	Mou P, Zeng Z, Li Q, Liu X, Xin X, WANNEMACHER K, et al. Identification of a calmodulin-binding domain in Sema4D that regulates its exodomain shedding in platelets. Blood. 2013;121:4221-30 pubmed publisher
western blot; Xenopus laevis	McCoy F, Darbandi R, Chen S, Eckard L, Dodd K, Jones K, et al. Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts. J Biol Chem. 2013;288:8838-48 pubmed publisher
western blot; human; fig 2	Dettmer U, Newman A, Luth E, Bartels T, Selkoe D. In vivo cross-linking reveals principally oligomeric forms of ?-synuclein and ?-synuclein in neurons and non-neural cells. J Biol Chem. 2013;288:6371-85 pubmed publisher
western blot; human; 1:1000	Makinoshima H, Ishii G, Kojima M, Fujii S, Higuchi Y, Kuwata T, et al. PTPRZ1 regulates calmodulin phosphorylation and tumor progression in small-cell lung carcinoma. BMC Cancer. 2012;12:537 pubmed publisher
immunocytochemistry; human; 1:2000	Aivar P, Fernandez Orth J, Gomis Pèrez C, Alberdi A, Alaimo A, Rodriguez M, et al. Surface expression and subunit specific control of steady protein levels by the Kv7.2 helix A-B linker. PLoS ONE. 2012;7:e47263 pubmed publisher
immunocytochemistry; rat; 1:5 western blot; rat; 1:1000	Swulius M, Kubota Y, Forest A, Waxham M. Structure and composition of the postsynaptic density during development. J Comp Neurol. 2010;518:4243-60 pubmed publisher
	Doroudi M, Plaisance M, Boyan B, Schwartz Z. Membrane actions of 1Î±,25(OH)2D3 are mediated by Ca(2+)/calmodulin-dependent protein kinase II in bone and cartilage cells. J Steroid Biochem Mol Biol. 2015;145:65-74 pubmed publisher
	Ulke Lemee A, Ishida H, Chappellaz M, Vogel H, MacDonald J. Two domains of the smoothelin-like 1 protein bind apo- and calcium-calmodulin independently. Biochim Biophys Acta. 2014;1844:1580-90 pubmed publisher
	Itakura M, Watanabe I, Sugaya T, Takahashi M. Direct association of the unique C-terminal tail of transmembrane AMPA receptor regulatory protein ?-8 with calcineurin. FEBS J. 2014;281:1366-78 pubmed publisher
	Maubach G, Sokolova O, Wolfien M, Rothkötter H, Naumann M. Ca2+/calmodulin-dependent kinase II contributes to inhibitor of nuclear factor-kappa B kinase complex activation in Helicobacter pylori infection. Int J Cancer. 2013;133:1507-12 pubmed publisher
	López Victorio C, Vélez Delvalle C, Beltrán Langarica A, Kuri Harcuch W. EDF-1 downregulates the CaM/Cn/NFAT signaling pathway during adipogenesis. Biochem Biophys Res Commun. 2013;432:146-51 pubmed publisher
	Lowry J, Brovkovych V, Zhang Y, Skidgel R. Endothelial nitric-oxide synthase activation generates an inducible nitric-oxide synthase-like output of nitric oxide in inflamed endothelium. J Biol Chem. 2013;288:4174-93 pubmed publisher
	Panina S, Stephan A, la Cour J, Jacobsen K, Kallerup L, Bumbuleviciute R, et al. Significance of calcium binding, tyrosine phosphorylation, and lysine trimethylation for the essential function of calmodulin in vertebrate cells analyzed in a novel gene replacement system. J Biol Chem. 2012;287:18173-81 pubmed publisher
	Dzijak R, Yildirim S, Kahle M, Novak P, Hnilicová J, Venit T, et al. Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner. PLoS ONE. 2012;7:e30529 pubmed publisher
	Ivanova M, Abner S, Pierce W, Klinge C. Ligand-dependent differences in estrogen receptor beta-interacting proteins identified in lung adenocarcinoma cells corresponds to estrogenic responses. Proteome Sci. 2011;9:60 pubmed publisher
	Pang Z, Cao P, Xu W, Sudhof T. Calmodulin controls synaptic strength via presynaptic activation of calmodulin kinase II. J Neurosci. 2010;30:4132-42 pubmed publisher
	Lai D, Wan M, Wu J, Preston Hurlburt P, Kushwaha R, Grundström T, et al. Induction of TLR4-target genes entails calcium/calmodulin-dependent regulation of chromatin remodeling. Proc Natl Acad Sci U S A. 2009;106:1169-74 pubmed publisher
	Flora J, Edmiston J, Secrist R, Li G, Rana G, Langston T, et al. Identification of in vitro differential cell secretions due to cigarette smoke condensate exposure using nanoflow capillary liquid chromatography and high-resolution mass spectrometry. Anal Bioanal Chem. 2008;391:2845-56 pubmed publisher
	Bish R, Myers M. Werner helicase-interacting protein 1 binds polyubiquitin via its zinc finger domain. J Biol Chem. 2007;282:23184-93 pubmed
	Hara T, Honda K, Shitashige M, Ono M, Matsuyama H, Naito K, et al. Mass spectrometry analysis of the native protein complex containing actinin-4 in prostate cancer cells. Mol Cell Proteomics. 2007;6:479-91 pubmed
	Kane S, Lienhard G. Calmodulin binds to the Rab GTPase activating protein required for insulin-stimulated GLUT4 translocation. Biochem Biophys Res Commun. 2005;335:175-80 pubmed

product information

Catalog Number :

05-173

Subcategory :

Cell Signaling

Product Name :

Anti-Calmodulin Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P62158

Host Name :

Mouse

Antigen :

Calmodulin

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Calmodulin Antibody

Cross Reactivity :

Bovine;Human;Mouse;Pig;Rat;Vertebrates

Background :

Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.

ALT Names :

calmodulin 1;calmodulin 1 (phosphorylase kinase, delta);phosphorylase kinase, delta subunit

Immunogen :

Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (128-148) of bovine calmodulin, conjugated with tyroglobulin.

Specificity :

Specific for both Ca2+-bound and Ca2+-free calmodulin, Mr 17 kDa. Does not recognize bovine S100-alpha, S100-beta, troponin C or parvalbumin.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at from date of receipt.