MDC1 Antibody, clone P2B11 | EMD Millipore 05-1572 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MDC1 Antibody, clone P2B11

catalog :

05-1572

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P2B11

The same clone is also sold as:

Invitrogen: MA5-27650
Abnova: MAB11472, MAB11467, MAB11468, MAB11471, MAB11474, MAB11463, MAB11477, MAB11470, MAB11464, MAB11460, MAB11462, MAB11476, MAB11465, MAB11461, MAB11475, MAB11466, MAB11473, MAB11469
Rockland Immunochemicals: 200-301-G12
LifeSpan Biosciences: LS-C231466, LS-C231467, LS-C231456, LS-C152962, LS-C231462, LS-C231461, LS-C231468, LS-C231452, LS-C231459, LS-C231453, LS-C150396, LS-C231464, LS-C231454, LS-C231457, LS-C231455, LS-C231458, LS-C231465, LS-C231460
StressMarq Biosciences: SMC-197D
OriGene: TA326493
Novus Biologicals: NBP2-12890

reactivity :

human, mouse, bovine, chimpanzee

application :

western blot, immunocytochemistry, western blot knockout validation

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; 1:1000; loading ...; fig 1g immunocytochemistry; human; 1:1000; loading ...; fig 1f	Nowsheen S, Aziz K, Luo K, Deng M, Qin B, Yuan J, et al. ZNF506-dependent positive feedback loop regulates H2AX signaling after DNA damage. Nat Commun. 2018;9:2736 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 2d	Gong Y, Handa N, Kowalczykowski S, de Lange T. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev. 2017;31:46-58 pubmed publisher
immunocytochemistry; human; 1:500; fig s1	Mackay D, Ullman K. ATR and a Chk1-Aurora B pathway coordinate postmitotic genome surveillance with cytokinetic abscission. Mol Biol Cell. 2015;26:2217-26 pubmed publisher
	Peng Y, Liao Q, Tan W, Peng C, Hu Z, Chen Y, et al. The deubiquitylating enzyme USP15 regulates homologous recombination repair and cancer cell response to PARP inhibitors. Nat Commun. 2019;10:1224 pubmed publisher
	Li Y, Luo K, Yin Y, Wu C, Deng M, Li L, et al. USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response. Nat Commun. 2017;8:15752 pubmed publisher
	Mayer A, Baran V, Sakakibara Y, Brzakova A, Ferencova I, Motlik J, et al. DNA damage response during mouse oocyte maturation. Cell Cycle. 2016;15:546-58 pubmed publisher

product information

Catalog Number :

05-1572

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-MDC1 Antibody, clone P2B11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q5PSV9

Host Name :

Mouse

Antigen :

MDC1

Clone :

P2B11

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-MDC1 Antibody, clone P2B11

Cross Reactivity :

Mouse;Human;Chimpanzee;Bovine

Background :

MDC1 is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.

ALT Names :

Nuclear factor with BRCT domains 1;homologue to Drosophila photoreceptor protein calphotin;mediator of DNA damage checkpoint 1

Immunogen :

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Specificity :

This antibody recognizes MDC1 at and around the N-terminus.

Package Size :

100 μg

Uses :

Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt