mTOR Antibody, clone 21A12.2 | EMD Millipore 05-1564 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

mTOR Antibody, clone 21A12.2

catalog :

05-1564

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

21A12.2

reactivity :

human

application :

western blot, immunocytochemistry

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1a	Douglas P, Ye R, Radhamani S, Cobban A, Jenkins N, Bartlett E, et al. Nocodazole-Induced Expression and Phosphorylation of Anillin and Other Mitotic Proteins Are Decreased in DNA-Dependent Protein Kinase Catalytic Subunit-Deficient Cells and Rescued by Inhibition of the Anaphase-Promoting Complex/Cyclosome with proTAME. Mol Cell Biol. 2020;40: pubmed publisher

product information

Catalog Number :

05-1564

Subcategory :

Cell Signaling

Product Name :

Anti-mTOR Antibody, clone 21A12.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q6R327

Host Name :

Mouse

Antigen :

mTOR

Clone :

clone 21A12.2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-mTOR Antibody, clone 21A12.2

Cross Reactivity :

Human

Background :

mTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 consists of at least mTOR, Raptor, and GL. mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473. TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

ALT Names :

TORC2-specific protein AVO3 ;rapamycin-insensitive companion of mTOR

Immunogen :

Full-length human mTOR

Specificity :

This antibody only detects mTOR.

Package Size :

100 µg

Uses :

Western Blotting;Immunocytochemistry

Storage :

Stable for 1 year at 2-8ºC from date of receipt.