PKCα Antibody, clone M4 | EMD Millipore 05-154 product information

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PKCα Antibody, clone M4

catalog :

05-154

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, neutralization, immunoprecipitation, radioimmunoassay, immunohistochemistry - frozen section, reverse phase protein lysate microarray, other

more info or order :

EMD Millipore product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 4a western blot; mouse; loading ...; fig 4b	Hunter D, Manglapus M, Bachay G, Claudepierre T, Dolan M, Gesuelli K, et al. CNS synapses are stabilized trans-synaptically by laminins and laminin-interacting proteins. J Comp Neurol. 2017;: pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig st6	Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig 3a	. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
western blot; human; loading ...	Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
other; mouse; 1:1000; fig s1	Chiang C, Uzoma I, Lane D, MemiÅ¡eviÄ‡ V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher
immunohistochemistry; mouse; 1:1,000	Johnson D, Donovan S, Dyer M. Mosaic deletion of Rb arrests rod differentiation and stimulates ectopic synaptogenesis in the mouse retina. J Comp Neurol. 2006;498:112-28 pubmed
	Kozuka T, Chaya T, Tamalu F, Shimada M, Fujimaki Aoba K, Kuwahara R, et al. The TRPM1 Channel Is Required for Development of the Rod ON Bipolar Cell-AII Amacrine Cell Pathway in the Retinal Circuit. J Neurosci. 2017;37:9889-9900 pubmed publisher
	Liu W, Cvekl A. Six3 in a small population of progenitors at E8.5 is required for neuroretinal specification via regulating cell signaling and survival in mice. Dev Biol. 2017;428:164-175 pubmed publisher
	Fujita H, Aoki H, Ajioka I, Yamazaki M, Abe M, Oh Nishi A, et al. Detailed expression pattern of aldolase C (Aldoc) in the cerebellum, retina and other areas of the CNS studied in Aldoc-Venus knock-in mice. PLoS ONE. 2014;9:e86679 pubmed publisher
	Hennessy B, Lu Y, Gonzalez Angulo A, Carey M, Myhre S, Ju Z, et al. A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers. Clin Proteomics. 2010;6:129-51 pubmed publisher
	de Melo J, Peng G, Chen S, Blackshaw S. The Spalt family transcription factor Sall3 regulates the development of cone photoreceptors and retinal horizontal interneurons. Development. 2011;138:2325-36 pubmed publisher
	Rapicavoli N, Poth E, Blackshaw S. The long noncoding RNA RNCR2 directs mouse retinal cell specification. BMC Dev Biol. 2010;10:49 pubmed publisher

product information

Catalog Number :

05-154

Subcategory :

Cell Signaling

Product Name :

Anti-PKCα Antibody, clone M4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P17252

Host Name :

Mouse

Antigen :

PKCα

Clone :

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-PKCα Antibody, clone M4

Cross Reactivity :

Human;Bovine;Mouse;Rat;Rabbit

Background :

Protein Kinase c alpha (PKC alpha) is a 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell-cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy-terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

ALT Names :

aging-associated gene 6;protein kinase C alpha type;protein kinase C, alpha

Immunogen :

PKCα purified from rabbit brain cytosol.

Specificity :

Specific for PKCα. MW: ~82 kDa.

Package Size :

100 μg

Uses :

Immunoprecipitation;Neutralizing;Radioimmunoassay;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.

more info or order :

EMD Millipore product webpage