NFĸB Antibody, p65, clone 1G10.2 | EMD Millipore 05-1469 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NFĸB Antibody, p65, clone 1G10.2

catalog :

05-1469

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1G10.2

reactivity :

human, mouse, bovine

application :

western blot, immunocytochemistry

citations: 32

Reference
Pernía M, Diaz I, Colmenárez Raga A, Rivadulla C, Cudeiro J, Plaza I, et al. Cross-modal reaction of auditory and visual cortices after long-term bilateral hearing deprivation in the rat. Brain Struct Funct. 2020;225:129-148 pubmed publisher
Thiebaud N, Gribble F, Reimann F, Trapp S, Fadool D. A unique olfactory bulb microcircuit driven by neurons expressing the precursor to glucagon-like peptide 1. Sci Rep. 2019;9:15542 pubmed publisher
Ueno H, Suemitsu S, Murakami S, Kitamura N, Wani K, Matsumoto Y, et al. Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices. IBRO Rep. 2019;6:1-17 pubmed publisher
Chang A, Liang Z, Dai H, Chapdelaine Williams A, Andrews N, Bronson R, et al. Neural blastocyst complementation enables mouse forebrain organogenesis. Nature. 2018;563:126-130 pubmed publisher
Corder K, Cortes M, Bartley A, Lear S, Lubin F, Dobrunz L. Prefrontal cortex-dependent innate behaviors are altered by selective knockdown of Gad1 in neuropeptide Y interneurons. PLoS ONE. 2018;13:e0200809 pubmed publisher
Vuong C, Wei W, Lee J, Lin C, Damianov A, de la Torre Ubieta L, et al. Rbfox1 Regulates Synaptic Transmission through the Inhibitory Neuron-Specific vSNARE Vamp1. Neuron. 2018;98:127-141.e7 pubmed publisher
Oda S, Tsuneoka Y, Yoshida S, Adachi Akahane S, Ito M, Kuroda M, et al. Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex. J Comp Neurol. 2018;526:1329-1350 pubmed publisher
Hernandez M, Namiranian P, Nguyen E, Fonseca M, Tenner A. C5a Increases the Injury to Primary Neurons Elicited by Fibrillar Amyloid Beta. ASN Neuro. 2017;9:1759091416687871 pubmed publisher
Dimidschstein J, Chen Q, Tremblay R, Rogers S, Saldi G, Guo L, et al. A viral strategy for targeting and manipulating interneurons across vertebrate species. Nat Neurosci. 2016;19:1743-1749 pubmed publisher
Benn A, Barker G, Stuart S, Roloff E, Teschemacher A, Warburton E, et al. Optogenetic Stimulation of Prefrontal Glutamatergic Neurons Enhances Recognition Memory. J Neurosci. 2016;36:4930-9 pubmed publisher
Agca S, Houen G, Trier N. Characterization of continuous B-cell epitopes in the N-terminus of glutamate decarboxylase67 using monoclonal antibodies. J Pept Sci. 2014;20:928-34 pubmed publisher
Gaven F, Marin P, Claeysen S. Primary culture of mouse dopaminergic neurons. J Vis Exp. 2014;:e51751 pubmed publisher
Zizioli D, Guarienti M, Tobia C, Gariano G, Borsani G, Bresciani R, et al. Molecular cloning and knockdown of galactocerebrosidase in zebrafish: new insights into the pathogenesis of Krabbe's disease. Biochim Biophys Acta. 2014;1842:665-75 pubmed publisher
Balu D, Takagi S, Puhl M, Benneyworth M, Coyle J. D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain. Cell Mol Neurobiol. 2014;34:419-35 pubmed publisher
Keeve P, Dittmar T, Gassmann G, Grimm W, Niggemann B, Friedmann A. Characterization and analysis of migration patterns of dentospheres derived from periodontal tissue and the palate. J Periodontal Res. 2013;48:276-85 pubmed publisher
Chapman R, Lall V, Maxeiner S, Willecke K, Deuchars J, King A. Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice. Brain Struct Funct. 2013;218:751-65 pubmed publisher
Gribaudo S, Bovetti S, Friard O, Denorme M, Oboti L, Fasolo A, et al. Transitory and activity-dependent expression of neurogranin in olfactory bulb tufted cells during mouse postnatal development. J Comp Neurol. 2012;520:3055-69 pubmed publisher
Ohishi T, Wang L, Akane H, Shiraki A, Goto K, Ikarashi Y, et al. Reversible aberration of neurogenesis affecting late-stage differentiation in the hippocampal dentate gyrus of rat offspring after maternal exposure to manganese chloride. Reprod Toxicol. 2012;34:408-19 pubmed publisher
Saegusa Y, Fujimoto H, Woo G, Ohishi T, Wang L, Mitsumori K, et al. Transient aberration of neuronal development in the hippocampal dentate gyrus after developmental exposure to brominated flame retardants in rats. Arch Toxicol. 2012;86:1431-42 pubmed publisher
Trifonov S, Houtani T, Kase M, Toida K, Maruyama M, Yamashita Y, et al. Lateral regions of the rodent striatum reveal elevated glutamate decarboxylase 1 mRNA expression in medium-sized projection neurons. Eur J Neurosci. 2012;35:711-22 pubmed publisher
Martin J, Brown A, Balkowiec A. Glia determine the course of brain-derived neurotrophic factor-mediated dendritogenesis and provide a soluble inhibitory cue to dendritic growth in the brainstem. Neuroscience. 2012;207:333-46 pubmed publisher
Kadriu B, Guidotti A, Chen Y, Grayson D. DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain. J Comp Neurol. 2012;520:1951-64 pubmed publisher
Stranahan A, Jiam N, Spiegel A, Gallagher M. Aging reduces total neuron number in the dorsal component of the rodent prefrontal cortex. J Comp Neurol. 2012;520:1318-26 pubmed publisher
Mészár Z, Girard F, Saper C, Celio M. The lateral hypothalamic parvalbumin-immunoreactive (PV1) nucleus in rodents. J Comp Neurol. 2012;520:798-815 pubmed publisher
Nouel D, Burt M, Zhang Y, Harvey L, Boksa P. Prenatal exposure to bacterial endotoxin reduces the number of GAD67- and reelin-immunoreactive neurons in the hippocampus of rat offspring. Eur Neuropsychopharmacol. 2012;22:300-7 pubmed publisher
Willett R, Greene L. Gata2 is required for migration and differentiation of retinorecipient neurons in the superior colliculus. J Neurosci. 2011;31:4444-55 pubmed publisher
Bøttger P, Tracz Z, Heuck A, Nissen P, Romero Ramos M, Lykke Hartmann K. Distribution of Na/K-ATPase alpha 3 isoform, a sodium-potassium P-type pump associated with rapid-onset of dystonia parkinsonism (RDP) in the adult mouse brain. J Comp Neurol. 2011;519:376-404 pubmed publisher
Kay R, Meyer E, Illig K, Brunjes P. Spatial distribution of neural activity in the anterior olfactory nucleus evoked by odor and electrical stimulation. J Comp Neurol. 2011;519:277-89 pubmed publisher
Ohishi T, Wang L, Ogawa B, Fujisawa K, Taniai E, Hayashi H, et al. No effect of sustained systemic growth retardation on the distribution of Reelin-expressing interneurons in the neuron-producing hippocampal dentate gyrus in rats. Reprod Toxicol. 2010;30:591-9 pubmed publisher
He Y, Hsuchou H, Wu X, Kastin A, Khan R, Pistell P, et al. Interleukin-15 receptor is essential to facilitate GABA transmission and hippocampal-dependent memory. J Neurosci. 2010;30:4725-34 pubmed publisher
Jiang Z, Belforte J, Lu Y, Yabe Y, Pickel J, Smith C, et al. eIF2alpha Phosphorylation-dependent translation in CA1 pyramidal cells impairs hippocampal memory consolidation without affecting general translation. J Neurosci. 2010;30:2582-94 pubmed publisher
Toyoshima M, Sakurai K, Shimazaki K, Takeda Y, Nakamoto M, Serizawa S, et al. Preferential localization of neural cell recognition molecule NB-2 in developing glutamatergic neurons in the rat auditory brainstem. J Comp Neurol. 2009;513:349-62 pubmed publisher

product information

Catalog Number :

05-1469

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-NFĸB Antibody, p65, clone 1G10.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P19838

Host Name :

Mouse

Antigen :

NFĸB

Clone :

1G10.2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-NFĸB Antibody, p65, clone 1G10.2

Cross Reactivity :

Human;Mouse;Rat;Bovine

Background :

NFĸB (Nuclear Factor kappa B) represents a group of transcription factors that are structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel. The five members in mammals includes Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100)1, 2. NFĸB/Rel proteins exist as homo- or heterodimer complexes. In a majority of unstimulated cells, the NFĸB transcription factors are bound to the inhibitory IĸB proteins and retained in the cytoplasm in their inactive form. Upon stimulation by multiple inducers, IĸBα is rapidly phosphorylated and degraded. This results in the release of the NFĸB complex, most commonly the p105/p65 heterodimer. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. The p50/p65 heteroduplex then translocates to the nucleus where it activates gene transcription.

Immunogen :

Synthetic peptide corresponding to the NLS region of human NFĸB p65.

Specificity :

Detects NFĸB p65.

Package Size :

200 μg

Uses :

Western Blotting;Immunofluorescence;Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.