Src Family (WTAPE) Antibody, clone 2E8.2 | EMD Millipore 05-1461 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Src Family (WTAPE) Antibody, clone 2E8.2

catalog :

05-1461

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2E8.2

reactivity :

human, mouse

application :

western blot

product information

Catalog Number :

05-1461

Subcategory :

Cell Signaling

Product Name :

Anti-Src Family (WTAPE) Antibody, clone 2E8.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12931

Host Name :

Mouse

Antigen :

Src Family (WTAPE)

Clone :

2E8.2

Conjugate :

Purified

Isotype :

IgG3κ

Product Description :

Anti-Src Family (WTAPE) Antibody, clone 2E8.2

Cross Reactivity :

Human;Mouse;Rat

Background :

pp60c-Src (Src) is the prototype non-receptor tyrosine kinase Src was the first proto-oncogenic protein discovered. The 9 members of the Src family include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk. They share common structural characteristics that include an N-terminal anchor sequence that allows the protein to the membrane followed by both an SH3 and an SH2 domain, a catalytic domain, an activation loop, and finally a C-term region that binds to the SH2 domain when phosphorylated (Tyr527 in Src that is phosphorylated by Csk). This binding causes Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target, and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype. Homology to Src in three regions is shared by a number of proteins, SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain.

ALT Names :

Integrin-linked kinase;59 kDa serine/threonine-protein kinase

Immunogen :

Shared Src family sequence found at the C-term. Amino acid sequence GAKFPIKWTAPE.

Specificity :

Recognizes Src Family Members at the following amino acids: Src:424-435, Lyn:402-413, Fyn:425-436, Yes:431-442, Lck:399-411, Hck:394-405, Blk:394-405, and Fgr: 417-428.

Package Size :

200 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance