SMAD1 Antibody, clone AS22 | EMD Millipore 05-1459 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

SMAD1 Antibody, clone AS22

catalog :

05-1459

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AS22

reactivity :

human, mouse, chicken

application :

western blot, immunocytochemistry

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; chicken; 1:50; fig 2	Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
western blot; mouse; 1:500 western blot; human; 1:500	Cruz A, Silva M, Caon T, Simões C. Addition of bone morphogenetic protein type 2 to ascorbate and β-glycerophosphate supplementation did not enhance osteogenic differentiation of human adipose-derived stem cells. J Appl Oral Sci. 2012;20:628-35 pubmed

product information

Catalog Number :

05-1459

Subcategory :

Cell Signaling

Product Name :

Anti-SMAD1 Antibody, clone AS22

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q15797

Host Name :

Mouse

Antigen :

SMAD1

Clone :

AS22

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-SMAD1 Antibody, clone AS22

Cross Reactivity :

Human;Mouse

Background :

Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

ALT Names :

SMAD 1;SMAD family member 1;SMAD, mothers against DPP homolog 1;MAD, mothers against decapentaplegic homolog 1;Mad-related protein 1;Mothers against DPP homolog 1;Sma- and Mad-related protein 1;TGF-beta signaling protein 1 ;Transforming growth factor-beta-signaling protein 1

Immunogen :

GST-fusion protein corresponding to amino acids 1-110 of human SMAD1.

Specificity :

Detects SMAD1

Package Size :

200 μg

Uses :

Western Blotting;Immunofluorescence

Storage :

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.