CD45 Antibody, clone F10-89-4 | EMD Millipore 05-1410 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CD45 Antibody, clone F10-89-4

catalog :

05-1410

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F10-89-4

The same clone is also sold as:

LifeSpan Biosciences: LS-C351653, LS-B5327, LS-C351658, LS-C351646, LS-C187603, LS-C351655, LS-C351654, LS-C351660, LS-C351656, LS-C351651, LS-C45412, LS-C188772
Santa Cruz Biotechnology: sc-52490
OriGene: SM1795APC, SM1795B, SM21APC, SM1795PS, SM1795BT, SM1795P, SM1795F, SM1795R, SM21FT, SM21PT
Novus Biologicals: NBP2-54455-100ug
Bio-Rad: MCA87P647, MCA87BT, MCA87APCT, MCA87A700, MCA87PECY5.5, MCA87, MCA87P750, MCA87EL, MCA87GA, MCA87PB, MCA87B, MCA87PBT, MCA87AMO, MCA87A647, MCA87A700T, MCA87FT, MCA87A647T, MCA87A488T, MCA87PE, MCA87F, MCA87T, MCA87SBV515, MCA87PERCP, MCA87APC, MCA87A488
Abcam: ab30470, ab197730
Caprico Biotechnologies: 101601, 101665, 1016125, 101625, 101635, 101645, 101615
Boster: FC00555-FITC
Invitrogen: A15457, MA5-16669, MA5-16670, MA1-80428
Abnova: MAB6070, MAB6069, MAB5626, MAB6071, MAB6066, MAB16148, MAB6067, MAB6068, MAB6065, MAB6064

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, activation, immunohistochemistry - paraffin section

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:100	Zhang Y, Lam O, Nguyen M, Ng G, Pear W, Ai W, et al. Mastermind-like transcriptional co-activator-mediated Notch signaling is indispensable for maintaining conjunctival epithelial identity. Development. 2013;140:594-605 pubmed publisher
	Mehdipour M, Etienne J, Chen C, Gathwala R, Rehman M, Kato C, et al. Rejuvenation of brain, liver and muscle by simultaneous pharmacological modulation of two signaling determinants, that change in opposite directions with age. Aging (Albany NY). 2019;11:5628-5645 pubmed publisher
	Kalakech H, Hibert P, Prunier Mirebeau D, Tamareille S, Letournel F, Macchi L, et al. RISK and SAFE signaling pathway involvement in apolipoprotein A-I-induced cardioprotection. PLoS ONE. 2014;9:e107950 pubmed publisher
	Yu J, Wang A, Tang Z, HENRY J, Li Ping Lee B, Zhu Y, et al. The effect of stromal cell-derived factor-1?/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration. Biomaterials. 2012;33:8062-74 pubmed publisher
	Preobrazhenska O, Wright J, Churg A. Regional heterogeneity in murine lung fibroblasts from normal mice or mice exposed once to cigarette smoke. PLoS ONE. 2012;7:e39761 pubmed publisher

product information

Catalog Number :

05-1410

Subcategory :

Cell Signaling

Product Name :

Anti-CD45 Antibody, clone F10-89-4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P08575

Host Name :

Mouse

Antigen :

CD45

Clone :

F10-89-4

Conjugate :

Affinity Purified

Isotype :

IgG2a

Product Description :

Anti-CD45 Antibody, clone F10-89-4

Cross Reactivity :

Human;Mouse;Rat

Background :

CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

ALT Names :

CD45 antigen;Leukocyte common antigen precursor;SCID due to PTPRC deficiency;T200 glycoprotein;T200 leukocyte common antigen;Human homolog of severe combined immunodeficiency due to PTPRC deficiency;Leukocyte-common antigen;Protein tyrosine phosphatase, receptor type, C;Protein tyrosine phosphatase, receptor type, c polypeptide

Immunogen :

Purified T cells from human lymph nodes

Specificity :

This antibody reacts with the leucocyte common antigen (LCA) complex to be found on lymphocytes, monocytes, granulocytes, thymocytes and malignant T and B cells. No reactivity has been observed with primary or metastatic carcinoma cells. Plasma cells or myeloma cells may have weak expression or be negative for this antigen. The LCA comprises of at least 5 isoforms ranging from 180-220 kDa which are produced as a result of alternative splicing of exons A, B or C. CBL 124 recognizes epitopes common to all of the isoforms. Antigen distribution: Thymocytes 95% Granulocytes 95% Monocytes 95% B cells (CD20+) 95% T cells (CD3+) 95% NK cells (CD16+) 95% Peripheral blood lymphocytes 95% FUSION PARTNER: NS1 myeloma cell line

Package Size :

100 µg

Uses :

Flow Cytometry;Western Blotting;Immunofluorescence;Immunoprecipitation;Immunohistochemistry (Paraffin)

Storage :

For use within 1 month of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.