Histone H3 Antibody, (Unmodified Lys4), clone CMA301 | EMD Millipore 05-1341 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Histone H3 Antibody, (Unmodified Lys4), clone CMA301

catalog :

05-1341

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CMA301

reactivity :

Saccharomycetales, human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:5000; fig 3	Penterling C, Drexler G, Böhland C, Stamp R, Wilke C, Braselmann H, et al. Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair. PLoS ONE. 2016;11:e0156599 pubmed publisher
western blot; human; 1:1000	Maroschik B, Gürtler A, Kramer A, Rößler U, Gomolka M, Hornhardt S, et al. Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines. Radiat Oncol. 2014;9:15 pubmed publisher
western blot; Saccharomycetales	Maltby V, Martin B, Brind Amour J, Chruscicki A, McBurney K, Schulze J, et al. Histone H3K4 demethylation is negatively regulated by histone H3 acetylation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2012;109:18505-10 pubmed publisher
	Liu X, Zhang W, Wu Z, Yang Y, Kang Y. Copper levels affect targeting of hypoxia-inducible factor 1α to the promoters of hypoxia-regulated genes. J Biol Chem. 2018;293:14669-14677 pubmed publisher
	Guo L, Wang X, Ren L, Zeng M, Wang S, Weng Y, et al. HBx affects CUL4-DDB1 function in both positive and negative manners. Biochem Biophys Res Commun. 2014;450:1492-7 pubmed publisher
	Kamarajan P, Shin J, Qian X, Matte B, Zhu J, Kapila Y. ADAM17-mediated CD44 cleavage promotes orasphere formation or stemness and tumorigenesis in HNSCC. Cancer Med. 2013;2:793-802 pubmed publisher

product information

Catalog Number :

05-1341

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Histone H3

Clone :

CMA301

Conjugate :

Affinity Purified

Isotype :

IgG2bκ

Product Description :

Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301

Cross Reactivity :

Human;Vertebrates

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

ALT Names :

H3 histone family, member T;histone 3, H3 ;histone cluster 3, H3

Immunogen :

Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, conjugated to KLH.

Specificity :

This antibody recognizes Histone H3 that is unmodified at Thr3 and Lys4 but does not recognize phosphorylated Thr3 nor acetylated or methylated Lys4. The antibody binding specificity allows modifications of Lys9, Ser10, and/or Lys141.

Package Size :

100 µg

Uses :

Western Blotting;Immunocytochemistry;ELISA;Immunoprecipitation;Flow Cytometry;Dot Blot

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.