Trimethyl Histone H3 (Lys4) Antibody, clone CMA304 | EMD Millipore 05-1339 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Trimethyl Histone H3 (Lys4) Antibody, clone CMA304

catalog :

05-1339

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CMA304

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

citations: 7

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human	Liefke R, Borggrefe T. Probing the epigenetic status at Notch target genes. Methods Mol Biol. 2014;1187:255-76 pubmed publisher
immunohistochemistry - paraffin section; human	Wang Z, Yang J, Wang Y, Lou J, Chen J, Liu C, et al. Decreased histone H2B monoubiquitination in malignant gastric carcinoma. World J Gastroenterol. 2013;19:8099-107 pubmed publisher
	Stojic L, Lun A, Mascalchi P, Ernst C, Redmond A, Mangei J, et al. A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division. Nat Commun. 2020;11:1851 pubmed publisher
	Żylicz J, Bousard A, Zumer K, Dossin F, Mohammad E, da Rocha S, et al. The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell. 2019;176:182-197.e23 pubmed publisher
	Aitken S, Ibarra Soria X, Kentepozidou E, Flicek P, Feig C, Marioni J, et al. CTCF maintains regulatory homeostasis of cancer pathways. Genome Biol. 2018;19:106 pubmed publisher
	Chang C, Yang X, Pursell B, Mercurio A. Id2 complexes with the SNAG domain of Snai1 inhibiting Snai1-mediated repression of integrin ?4. Mol Cell Biol. 2013;33:3795-804 pubmed publisher
	Inoue A, Matoba S, Zhang Y. Transcriptional activation of transposable elements in mouse zygotes is independent of Tet3-mediated 5-methylcytosine oxidation. Cell Res. 2012;22:1640-9 pubmed publisher

product information

Catalog Number :

05-1339

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Trimethyl Histone H3 (Lys4)

Clone :

CMA304

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304

Cross Reactivity :

Human;Vertebrates

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H3 (Lys4) is is found at the 5' end of active genes and contributes to transcriptional activation by recruiting chromatin-remodelling enzymes. Accordingly, the inactive X chromosome is largely devoid of this mark.

ALT Names :

H3K4me3;Histone H3 (tri methyl K4);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, trimethylated on Lys4, conjugated to KLH.

Specificity :

This antibody specifically recognizes Histone H3 trimethylated at Lys4. The antibody binding specificity allows for modifications of Lys91.

Package Size :

100 µg

Uses :

ELISA;Immunocytochemistry;Immunoprecipitation;Western Blotting;Multiplexing;Dot Blot

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.