Dimethyl Histone H3 (Lys4) Antibody, clone CMA303 | EMD Millipore 05-1338 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Dimethyl Histone H3 (Lys4) Antibody, clone CMA303

catalog :

05-1338

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CMA303

reactivity :

human, mouse, brewer's yeast, fruit fly , roundworm

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, proximity ligation assay

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3a western blot; mouse; loading ...; fig 3a	Zhong J, Li X, Cai W, Wang Y, Dong S, Yang J, et al. TET1 modulates H4K16 acetylation by controlling auto-acetylation of hMOF to affect gene regulation and DNA repair function. Nucleic Acids Res. 2017;45:672-684 pubmed publisher
chromatin immunoprecipitation; brewer's yeast; fig 5	Jones J, Singh P, Govind C. Recruitment of Saccharomyces cerevisiae Cmr1/Ydl156w to Coding Regions Promotes Transcription Genome Wide. PLoS ONE. 2016;11:e0148897 pubmed publisher
immunocytochemistry; roundworm	Vandamme J, Sidoli S, Mariani L, Friis C, Christensen J, Helin K, et al. H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans. Nucleic Acids Res. 2015;43:9694-710 pubmed publisher
proximity ligation assay; human; fig 3	Shankman L, Gomez D, Cherepanova O, Salmon M, Alencar G, Haskins R, et al. KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis. Nat Med. 2015;21:628-37 pubmed publisher
chromatin immunoprecipitation; human	Liefke R, Borggrefe T. Probing the epigenetic status at Notch target genes. Methods Mol Biol. 2014;1187:255-76 pubmed publisher
immunohistochemistry - paraffin section; human	Wang Z, Yang J, Wang Y, Lou J, Chen J, Liu C, et al. Decreased histone H2B monoubiquitination in malignant gastric carcinoma. World J Gastroenterol. 2013;19:8099-107 pubmed publisher
chromatin immunoprecipitation; fruit fly	Meier K, Mathieu E, Finkernagel F, Reuter L, Scharfe M, Doehlemann G, et al. LINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes. PLoS Genet. 2012;8:e1002676 pubmed publisher
	Mukai J, Cannavó E, Crabtree G, Sun Z, Diamantopoulou A, Thakur P, et al. Recapitulation and Reversal of Schizophrenia-Related Phenotypes in Setd1a-Deficient Mice. Neuron. 2019;104:471-487.e12 pubmed publisher
	Kim H, Beese Sims S, COLAIACOVO M. Fanconi Anemia FANCM/FNCM-1 and FANCD2/FCD-2 Are Required for Maintaining Histone Methylation Levels and Interact with the Histone Demethylase LSD1/SPR-5 in Caenorhabditis elegans. Genetics. 2018;209:409-423 pubmed publisher
	Huggins I, Bos T, Gaylord O, Jessen C, Lonquich B, Puranen A, et al. The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells. Nat Commun. 2017;8:1034 pubmed publisher
	Shao G, Chen J, Zhang L, Huang P, Lu H, Jin J, et al. Dynamic patterns of histone H3 lysine 4 methyltransferases and demethylases during mouse preimplantation development. In Vitro Cell Dev Biol Anim. 2014;50:603-13 pubmed publisher
	Lim H, Dimova N, Tan M, Sigoillot F, King R, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013;33:4166-80 pubmed publisher
	Shi L, Cui S, Engel J, Tanabe O. Lysine-specific demethylase 1 is a therapeutic target for fetal hemoglobin induction. Nat Med. 2013;19:291-4 pubmed publisher

product information

Catalog Number :

05-1338

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Dimethyl Histone H3 (Lys4)

Clone :

CMA303

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303

Cross Reactivity :

Human;Vertebrates

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Dimethyl-lysine 4 histone H3 (H3K4me2) is a transcription-activating chromatin mark, and dimethyl histone H3 Lys4 (H3K4me2) is depleted from regions with DNA methylation. Multipotential hematopoietic cells have a subset of genes that are differentially methylated (H3K4me2+/me3-). These genes are transcriptionally silent and highly enriched in lineage-specific hematopoietic genes, suggesting a role for H3K4 methylation in differentiation.

ALT Names :

H3K4me2;Histone H3 (di methyl K4);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, dimethylated on Lys4, conjugated to KLH.

Specificity :

This antibody specifically recognizes Histone H3 dimethylated at Lys4. The antibody binding specificity allows for phosphorylation of Thr3 and/or modifications of Lys91.

Package Size :

100 µg

Uses :

Western Blotting;Immunoprecipitation;Dot Blot;Immunocytochemistry;ELISA;Multiplexing;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.