Dimethyl Histone H3 (Lys4) Antibody, clone CMA303, Trial Size | EMD Millipore 05-1338-S product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Dimethyl Histone H3 (Lys4) Antibody, clone CMA303, Trial Size

catalog :

05-1338-S

quantity :

10 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CMA303

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, proximity ligation assay

citations: 4

Published Application/Species/Sample/Dilution	Reference
proximity ligation assay; human; fig 3	Shankman L, Gomez D, Cherepanova O, Salmon M, Alencar G, Haskins R, et al. KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis. Nat Med. 2015;21:628-37 pubmed publisher
	Kim H, Beese Sims S, COLAIACOVO M. Fanconi Anemia FANCM/FNCM-1 and FANCD2/FCD-2 Are Required for Maintaining Histone Methylation Levels and Interact with the Histone Demethylase LSD1/SPR-5 in Caenorhabditis elegans. Genetics. 2018;209:409-423 pubmed publisher
	Lim H, Dimova N, Tan M, Sigoillot F, King R, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013;33:4166-80 pubmed publisher
	Meier K, Mathieu E, Finkernagel F, Reuter L, Scharfe M, Doehlemann G, et al. LINT, a novel dL(3)mbt-containing complex, represses malignant brain tumour signature genes. PLoS Genet. 2012;8:e1002676 pubmed publisher

product information

Catalog Number :

05-1338-S

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303, Trial Size

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Dimethyl Histone H3 (Lys4)

Clone :

CMA303

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303, Trial Size

Cross Reactivity :

Human;Vertebrates

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Dimethyl-lysine 4 histone H3 (H3K4me2) is a transcription-activating chromatin mark, and dimethyl histone H3 Lys4 (H3K4me2) is depleted from regions with DNA methylation. Multipotential hematopoietic cells have a subset of genes that are differentially methylated (H3K4me2+/me3-). These genes are transcriptionally silent and highly enriched in lineage-specific hematopoietic genes, suggesting a role for H3K4 methylation in differentiation.

ALT Names :

H3K4me2;Histone H3 (di methyl K4);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, dimethylated on Lys4, conjugated to KLH.

Specificity :

This antibody specifically recognizes Histone H3 dimethylated at Lys4. The antibody binding specificity allows for phosphorylation of Thr3 and/or modifications of Lys91.

Package Size :

10 µg

Uses :

Western Blotting;Chromatin Immunoprecipitation (ChIP);Immunocytochemistry;Immunoprecipitation;Multiplexing;Dot Blot;ELISA

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.