phospho-Histone H3 (Ser10) Antibody, clone CMA312 | EMD Millipore 05-1336 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Histone H3 (Ser10) Antibody, clone CMA312

catalog :

05-1336

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CMA312

reactivity :

human, mouse, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; loading ...; fig s11	Li X, Song N, Liu L, Liu X, Ding X, Song X, et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun. 2017;8:14866 pubmed publisher
flow cytometry; mouse; 1:1000; fig s5b	Deluz C, Friman E, Strebinger D, Benke A, Raccaud M, Callegari A, et al. A role for mitotic bookmarking of SOX2 in pluripotency and differentiation. Genes Dev. 2016;30:2538-2550 pubmed
immunohistochemistry; mouse; 1:1000; loading ...; fig 4	Alexovič Matiašová A, Sevc J, Tomori Z, Gombalová Z, Gedrová S, Daxnerova Z. Quantitative analyses of cellularity and proliferative activity reveals the dynamics of the central canal lining during postnatal development of the rat. J Comp Neurol. 2017;525:693-707 pubmed publisher
western blot; mouse; 1:1000; fig 5	Badal S, Wang Y, Long J, Corcoran D, CHANG B, Truong L, et al. miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy. Nat Commun. 2016;7:12076 pubmed publisher
immunohistochemistry; chicken; 1:1000; fig s4G	Schock E, Chang C, Struve J, Chang Y, Chang J, Delany M, et al. Using the avian mutant talpid2 as a disease model for understanding the oral-facial phenotypes of oral-facial-digital syndrome. Dis Model Mech. 2015;8:855-66 pubmed publisher
	Lee J, Jung E, Heur M. Fibroblast growth factor 2 induces proliferation and fibrosis via SNAI1-mediated activation of CDK2 and ZEB1 in corneal endothelium. J Biol Chem. 2018;293:3758-3769 pubmed publisher
	Freund M, Walther T, von Bohlen und Halbach O. Effects of the angiotensin-(1-7) receptor Mas on cell proliferation and on the population of doublecortin positive cells within the dentate gyrus and the piriform cortex. Eur Neuropsychopharmacol. 2014;24:302-8 pubmed publisher
	Al Dhaheri Y, Eid A, Abuqamar S, Attoub S, Khasawneh M, Aiche G, et al. Mitotic arrest and apoptosis in breast cancer cells induced by Origanum majorana extract: upregulation of TNF-? and downregulation of survivin and mutant p53. PLoS ONE. 2013;8:e56649 pubmed publisher
	Al Dhaheri Y, Attoub S, Arafat K, Abuqamar S, Eid A, Al Faresi N, et al. Salinomycin induces apoptosis and senescence in breast cancer: upregulation of p21, downregulation of survivin and histone H3 and H4 hyperacetylation. Biochim Biophys Acta. 2013;1830:3121-35 pubmed publisher

product information

Catalog Number :

05-1336

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P84243

Host Name :

Mouse

Antigen :

phospho-Histone H3 (Ser10)

Clone :

CMA312

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312

Cross Reactivity :

Human;Vertebrates

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

ALT Names :

H3S10P;Histone H3 (phospho S10);H3 histone, family 3A

Immunogen :

Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, phosphorylated on Ser10, conjugated to KLH.

Specificity :

This antibody specifically recognizes Histone H3 phosphorylated at Ser10.

Package Size :

100 µg

Uses :

Western Blotting;ELISA;Immunocytochemistry;Multiplexing

Storage :

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.