Bmi-1 Antibody, clone DC9 | EMD Millipore 05-1322 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Bmi-1 Antibody, clone DC9

catalog :

05-1322

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DC9

The same clone is also sold as:

Cell Signaling Technology: 5855
Active Motif: 61089

reactivity :

human, mouse

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 4d western blot; human; loading ...; fig 4c	Zheng S, Hedl M, Abraham C. Twist1 and Twist2 Contribute to Cytokine Downregulation following Chronic NOD2 Stimulation of Human Macrophages through the Coordinated Regulation of Transcriptional Repressors and Activators. J Immunol. 2015;195:217-26 pubmed publisher
western blot; human	Ratovitski E. Phospho-?Np63?/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas. Cell Cycle. 2014;13:749-61 pubmed publisher
	Toth Z, Brulois K, Lee H, Izumiya Y, Tepper C, Kung H, et al. Biphasic euchromatin-to-heterochromatin transition on the KSHV genome following de novo infection. PLoS Pathog. 2013;9:e1003813 pubmed publisher

product information

Catalog Number :

05-1322

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Bmi-1 Antibody, clone DC9

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35226

Host Name :

Mouse

Antigen :

Bmi-1

Clone :

DC9

Conjugate :

Ascites

Isotype :

IgG1κ

Product Description :

Anti-Bmi-1 Antibody, clone DC9

Cross Reactivity :

Human;Mouse

Background :

The Bmi-1 protein is a member of the polycomb group (PcG) proteins. Bmi-1 has been reported as an oncogene by regulating the cell cycle inhibitors p16 and p19. Bmi-1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi-1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi-1 may regulate the self-renewal of other types of somatic stem cells, and it is also thought to inhibit aging in neurons through the suppression of p53. Several lines of evidence implicate Bmi-1 in tumorigenesis. Differential expression of Bmi-1 in a mouse model of metastatic prostate cancer vs. wild-type suggests Bmi-1 expression levels may predict response to therapy and survival in multiple types of cancer.

ALT Names :

B lymphoma Mo-MLV insertion region 1 homolog ;B lymphoma Mo-MLV insertion region 1 homolog (mouse);BMI1 polycomb ring finger oncogene ;Polycomb group RING finger protein 4;RING finger protein 51;murine leukemia viral (bmi-1) oncogene homolog;oncogene BMI-1;polycomb group ring finger 4

Immunogen :

Recombinant fusion protein of full length murine BMI-1 tagged with MBP.

Specificity :

This antibody recognizes murine BMI-1 (~35 kDa).

Package Size :

100 µg

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.