BMI1 Antibody, clone AF27 | EMD Millipore 05-1321 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BMI1 Antibody, clone AF27

catalog :

05-1321

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AF27

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human	Wu H, Balsbaugh J, Chandler H, Georgilis A, Zullow H, Shabanowitz J, et al. Mitogen-activated protein kinase signaling mediates phosphorylation of polycomb ortholog Cbx7. J Biol Chem. 2013;288:36398-408 pubmed publisher
	Gao R, Chen S, Kobayashi M, Yu H, Zhang Y, Wan Y, et al. Bmi1 promotes erythroid development through regulating ribosome biogenesis. Stem Cells. 2015;33:925-38 pubmed publisher
	van den Boom V, Rozenveld Geugien M, Bonardi F, Malanga D, van Gosliga D, Heijink A, et al. Nonredundant and locus-specific gene repression functions of PRC1 paralog family members in human hematopoietic stem/progenitor cells. Blood. 2013;121:2452-61 pubmed publisher
	Mao L, Ding J, Perdue A, Yang L, Zha Y, Ren M, et al. Cyclin E1 is a common target of BMI1 and MYCN and a prognostic marker for neuroblastoma progression. Oncogene. 2012;31:3785-95 pubmed publisher

product information

Catalog Number :

05-1321

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BMI1 Antibody, clone AF27

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35226

Host Name :

Mouse

Antigen :

BMI1

Clone :

AF27

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-BMI1 Antibody, clone AF27

Cross Reactivity :

Human

Background :

The BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.

ALT Names :

B lymphoma Mo-MLV insertion region 1 homolog ;B lymphoma Mo-MLV insertion region 1 homolog (mouse);BMI1 polycomb ring finger oncogene ;Polycomb group RING finger protein 4;RING finger protein 51 ;murine leukemia viral (bmi-1) oncogene homolog ;oncogene BMI-1 ;polycomb group protein Bmi1 ;polycomb group ring finger 4

Immunogen :

MBP-tagged recombinant protein corres-ponding to full length human BMI1.

Specificity :

This antibody recognizes BMI1.

Package Size :

100 µg

Uses :

Immunoprecipitation;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable at 2-8°C for 1 year from date of receipt.