EED Antibody, clone AA19 | EMD Millipore 05-1320 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EED Antibody, clone AA19

catalog :

05-1320

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AA19

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 4	Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun. 2016;7:11903 pubmed publisher
chromatin immunoprecipitation; human; 1:100 immunoprecipitation; human; 1:1000 western blot; human; 1:1000	Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, et al. The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127 pubmed publisher
immunocytochemistry; human	Natsume A, Ito M, Katsushima K, Ohka F, Hatanaka A, Shinjo K, et al. Chromatin regulator PRC2 is a key regulator of epigenetic plasticity in glioblastoma. Cancer Res. 2013;73:4559-70 pubmed publisher
	Sun B, Chang E, Gerhartl A, Szele F. Polycomb Protein Eed is Required for Neurogenesis and Cortical Injury Activation in the Subventricular Zone. Cereb Cortex. 2018;28:1369-1382 pubmed publisher
	Zhang Y, Yang X, Gui B, Xie G, Zhang D, Shang Y, et al. Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27. J Biol Chem. 2011;286:42414-25 pubmed publisher

product information

Catalog Number :

05-1320

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-EED Antibody, clone AA19

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O75530

Host Name :

Mouse

Antigen :

EED

Clone :

AA19

Conjugate :

Ascites

Isotype :

IgG2aκ

Product Description :

Anti-EED Antibody, clone AA19

Cross Reactivity :

Human;Mouse

Background :

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.

ALT Names :

WD protein associating with integrin cytoplasmic tails 1 ;embryonic ectoderm development

Immunogen :

Recombinant fusion protein of full length murine EED tagged with MBP.

Specificity :

This antibody recognizes mouse EED, (~30-65 kDa).

Package Size :

100 µg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.