EZH2 Antibody, clone BD43 | EMD Millipore 05-1319 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EZH2 Antibody, clone BD43

catalog :

05-1319

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BD43

reactivity :

human, mouse

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 2c	Ueda T, Nakata Y, Nagamachi A, Yamasaki N, Kanai A, Sera Y, et al. Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression. Proc Natl Acad Sci U S A. 2016;113:10370-5 pubmed publisher
immunoprecipitation; human; fig 5b western blot; human; fig 5d	Beltran M, Aparicio Prat E, Mazzolini R, Millanes Romero A, MassÃ³ P, Jenner R, et al. Splicing of a non-coding antisense transcript controls LEF1 gene expression. Nucleic Acids Res. 2015;43:5785-97 pubmed publisher
western blot; human	Hung S, Mody H, Marrache S, Bhutia Y, DAVIS F, Cho J, et al. Pharmacological reversal of histone methylation presensitizes pancreatic cancer cells to nucleoside drugs: in vitro optimization and novel nanoparticle delivery studies. PLoS ONE. 2013;8:e71196 pubmed publisher
chromatin immunoprecipitation; mouse	Hernández Hernández J, Mallappa C, Nasipak B, Oesterreich S, Imbalzano A. The Scaffold attachment factor b1 (Safb1) regulates myogenic differentiation by facilitating the transition of myogenic gene chromatin from a repressed to an activated state. Nucleic Acids Res. 2013;41:5704-16 pubmed publisher
	Battistelli C, Cicchini C, Santangelo L, Tramontano A, Grassi L, Gonzalez F, et al. The Snail repressor recruits EZH2 to specific genomic sites through the enrollment of the lncRNA HOTAIR in epithelial-to-mesenchymal transition. Oncogene. 2017;36:942-955 pubmed publisher
	Jene Sanz A, Váraljai R, Vilkova A, Khramtsova G, Khramtsov A, Olopade O, et al. Expression of polycomb targets predicts breast cancer prognosis. Mol Cell Biol. 2013;33:3951-61 pubmed publisher
	Dietrich N, Lerdrup M, Landt E, Agrawal Singh S, Bak M, Tommerup N, et al. REST-mediated recruitment of polycomb repressor complexes in mammalian cells. PLoS Genet. 2012;8:e1002494 pubmed publisher
	So A, Jung J, Lee S, Kim H, Kang K. DNA methyltransferase controls stem cell aging by regulating BMI1 and EZH2 through microRNAs. PLoS ONE. 2011;6:e19503 pubmed publisher
	Shi X, Zhang Y, Zhou X, Lu J, Guo Z, Huang P. Hepatitis B virus X protein regulates the mEZH2 promoter via the E2F1-binding site in AML12 cells. Chin J Cancer. 2011;30:273-9 pubmed
	Negishi M, Saraya A, Mochizuki S, Helin K, Koseki H, Iwama A. A novel zinc finger protein Zfp277 mediates transcriptional repression of the Ink4a/arf locus through polycomb repressive complex 1. PLoS ONE. 2010;5:e12373 pubmed publisher

product information

Catalog Number :

05-1319

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-EZH2 Antibody, clone BD43

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q15910

Host Name :

Mouse

Antigen :

EZH2

Clone :

BD43

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-EZH2 Antibody, clone BD43

Cross Reactivity :

Human;Mouse

Background :

EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene

ALT Names :

Enhancer of zeste homolog 2;Lysine N-methyltransferase 6 ;enhancer of zeste 2;enhancer of zeste homolog 2 ;ENX-1

Immunogen :

Recombinant fusion protein of amino acids 1-286 of human EZH2 tagged with MBP.

Specificity :

This antibody recognizes human EZH2 (~98kDa).

Package Size :

100 µg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.