Ubiquityl-Histone H2B Antibody, clone 56 | EMD Millipore 05-1312 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Ubiquityl-Histone H2B Antibody, clone 56

catalog :

05-1312

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, chicken, fruit fly

application :

western blot, immunohistochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, ChIP-Seq, other

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 8a	So C, Ramachandran S, Martin A. E3 Ubiquitin Ligases RNF20 and RNF40 Are Required for Double-Stranded Break (DSB) Repair: Evidence for Monoubiquitination of Histone H2B Lysine 120 as a Novel Axis of DSB Signaling and Repair. Mol Cell Biol. 2019;39: pubmed publisher
other; human; loading ...; fig 4c	Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
western blot; human; 1:1000	Guo X, Wang X, Wang Z, Banerjee S, Yang J, Huang L, et al. Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis. Nat Cell Biol. 2016;18:202-12 pubmed publisher
western blot; human; fig 4	Yu Z, Huang Y, Shieh S. Requirement for human Mps1/TTK in oxidative DNA damage repair and cell survival through MDM2 phosphorylation. Nucleic Acids Res. 2016;44:1133-50 pubmed publisher
ChIP-Seq; mouse; fig 4a western blot; mouse; fig 1b immunohistochemistry; human; fig 7a	Yao X, Tang Z, Fu X, Yin J, Liang Y, Li C, et al. The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination. EMBO J. 2015;34:2885-902 pubmed publisher
western blot; mouse	Ouyang S, Song Y, Tian Y, Chen Y, Yu X, Wang D. RNF8 deficiency results in neurodegeneration in mice. Neurobiol Aging. 2015;36:2850-2860 pubmed publisher
western blot; human; fig 4b	Cao Q, Yamamoto J, Isobe T, Tateno S, Murase Y, Chen Y, et al. Characterization of the Human Transcription Elongation Factor Rtf1: Evidence for Nonoverlapping Functions of Rtf1 and the Paf1 Complex. Mol Cell Biol. 2015;35:3459-70 pubmed publisher
immunohistochemistry - paraffin section; fruit fly	Mohan R, Dialynas G, Weake V, Liu J, Martin Brown S, Florens L, et al. Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration. Genes Dev. 2014;28:259-72 pubmed publisher
western blot; human; fig 3	Bedi U, Scheel A, Hennion M, Begus Nahrmann Y, Ruschoff J, Johnsen S. SUPT6H controls estrogen receptor activity and cellular differentiation by multiple epigenomic mechanisms. Oncogene. 2015;34:465-73 pubmed publisher
immunohistochemistry - paraffin section; human	Wang Z, Yang J, Wang Y, Lou J, Chen J, Liu C, et al. Decreased histone H2B monoubiquitination in malignant gastric carcinoma. World J Gastroenterol. 2013;19:8099-107 pubmed publisher
chromatin immunoprecipitation; chicken	Kohler K, McDonald J, Duke J, Arakawa H, Tan S, Kleinstein S, et al. Identification of core DNA elements that target somatic hypermutation. J Immunol. 2012;189:5314-26 pubmed publisher
	Li C, Irrazabal T, So C, Berru M, Du L, Lam E, et al. The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining. Nat Commun. 2018;9:1006 pubmed publisher
	Fereres S, SimÃ³n R, Mohd Sarip A, Verrijzer C, Busturia A. dRYBP counteracts chromatin-dependent activation and repression of transcription. PLoS ONE. 2014;9:e113255 pubmed publisher
	Sylvestersen K, Horn H, Jungmichel S, Jensen L, Nielsen M. Proteomic analysis of arginine methylation sites in human cells reveals dynamic regulation during transcriptional arrest. Mol Cell Proteomics. 2014;13:2072-88 pubmed publisher
	Kari V, Shchebet A, Neumann H, Johnsen S. The H2B ubiquitin ligase RNF40 cooperates with SUPT16H to induce dynamic changes in chromatin structure during DNA double-strand break repair. Cell Cycle. 2011;10:3495-504 pubmed publisher
	Weake V, Dyer J, Seidel C, Box A, Swanson S, Peak A, et al. Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. Genes Dev. 2011;25:1499-509 pubmed publisher
	Kim J, Roeder R. Nucleosomal H2B ubiquitylation with purified factors. Methods. 2011;54:331-8 pubmed publisher
	Borchert G, Holton N, Edwards K, Vogel L, Larson E. Histone H2A and H2B are monoubiquitinated at AID-targeted loci. PLoS ONE. 2010;5:e11641 pubmed publisher
	van der Knaap J, Kozhevnikova E, Langenberg K, Moshkin Y, Verrijzer C. Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. Mol Cell Biol. 2010;30:736-44 pubmed publisher

product information

Catalog Number :

05-1312

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Ubiquityl-Histone H2B Antibody, clone 56

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P62807

Host Name :

Mouse

Antigen :

Ubiquityl-Histone H2B

Clone :

clone 56

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-Ubiquityl-Histone H2B Antibody, clone 56

Cross Reactivity :

Human;Mouse;Rat

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression of several yeast genes.

ALT Names :

H2BUb;Histone H2B (ubiquityl);HIST1H2B

Immunogen :

Immunogen used was a branched peptide corresponding to the ubiquitination site on human H2B.

Specificity :

Recognizes histone H2B when it is ubiquitinated at Lys120 but not H2B in its unmodified state, or ubiquitin unconjugated to H2B. Mr ~25 kDa.

Package Size :

100 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.