Trimethyl Histone H3(Lys9) Antibody, clone CMA308 | EMD Millipore 05-1250 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Trimethyl Histone H3(Lys9) Antibody, clone CMA308

catalog :

05-1250

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CMA308

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:2000; fig 8	Mikolcevic P, Isoda M, Shibuya H, del Barco Barrantes I, Igea A, Suja J, et al. Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope. Nat Commun. 2016;7:11084 pubmed publisher
chromatin immunoprecipitation; human	Liefke R, Borggrefe T. Probing the epigenetic status at Notch target genes. Methods Mol Biol. 2014;1187:255-76 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Ivanauskiene K, Delbarre E, McGhie J, Küntziger T, Wong L, Collas P. The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity. Genome Res. 2014;24:1584-94 pubmed publisher
immunohistochemistry; human; 1:500	Verver D, Langedijk N, Jordan P, Repping S, Hamer G. The SMC5/6 complex is involved in crucial processes during human spermatogenesis. Biol Reprod. 2014;91:22 pubmed publisher
chromatin immunoprecipitation; human; 1:400	Oppenheimer H, Kumar A, Meir H, Schwartz I, Zini A, Haze A, et al. Set7/9 impacts COL2A1 expression through binding and repression of SirT1 histone deacetylation. J Bone Miner Res. 2014;29:348-60 pubmed publisher
	Legartova S, Jugova A, Stixova L, Kozubek S, Fojtova M, Zdrahal Z, et al. Epigenetic aspects of HP1 exchange kinetics in apoptotic chromatin. Biochimie. 2013;95:167-79 pubmed publisher
	Liu X, Yu C, Duan J, Luo M, Wang K, Tian G, et al. HDA6 directly interacts with DNA methyltransferase MET1 and maintains transposable element silencing in Arabidopsis. Plant Physiol. 2012;158:119-29 pubmed publisher

product information

Catalog Number :

05-1250

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Trimethyl Histone H3(Lys9)

Clone :

CMA308

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308

Cross Reactivity :

Human;Vertebrates

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.

ALT Names :

H3K9me3;Histone H3 (tri methyl K9);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, trimethylated on Lys9, conjugated to KLH.

Specificity :

This antibody recognizes Histone H3 trimethylated at Lys9. Some cross-reactivity with dimethyl Lys9 is detected at higher concentrations, but the difference in specificity is 27-fold1. Phosphorylation of Ser10 interferes with antibody binding to trimethyl Lys91.

Package Size :

100 µg

Uses :

Western Blotting;ELISA;Immunocytochemistry;Immunoprecipitation;Multiplexing

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.