Dimethyl Histone H3 (Lys9) Antibody, clone CMA307 | EMD Millipore 05-1249 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Dimethyl Histone H3 (Lys9) Antibody, clone CMA307

catalog :

05-1249

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CMA307

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 7

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 2f	Xia L, Huang W, Bellani M, Seidman M, Wu K, Fan D, et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017;31:653-668.e7 pubmed publisher
chromatin immunoprecipitation; human; fig 1	Bieberstein N, Kozáková E, Huranová M, Thakur P, Krchňáková Z, Krausova M, et al. TALE-directed local modulation of H3K9 methylation shapes exon recognition. Sci Rep. 2016;6:29961 pubmed publisher
chromatin immunoprecipitation; human; fig 8	Sarma P, Bag I, Ramaiah M, Kamal A, Bhadra U, Pal Bhadra M. Bisindole-PBD regulates breast cancer cell proliferation via SIRT-p53 axis. Cancer Biol Ther. 2015;16:1486-501 pubmed publisher
western blot; human; 0.5 ug/ml	Egelhofer T, Minoda A, Klugman S, Lee K, Kolasinska Zwierz P, Alekseyenko A, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18:91-3 pubmed publisher
	Choi J, Yoon S, Kim S, Ahn Jo S. KDM4B histone demethylase and G9a regulate expression of vascular adhesion proteins in cerebral microvessels. Sci Rep. 2017;7:45005 pubmed publisher
	Lin X, Tirichine L, Bowler C. Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species. Plant Methods. 2012;8:48 pubmed publisher
	Inoue A, Matoba S, Zhang Y. Transcriptional activation of transposable elements in mouse zygotes is independent of Tet3-mediated 5-methylcytosine oxidation. Cell Res. 2012;22:1640-9 pubmed publisher

product information

Catalog Number :

05-1249

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Dimethyl Histone H3 (Lys9)

Clone :

CMA307

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307

Cross Reactivity :

Human;Vertebrates

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.

ALT Names :

H3K4me2;Histone H3 (di methyl K4);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, dimethylated on Lys9, conjugated to KLH.

Specificity :

This antibody recognizes Histone H3 dimethylated at Lys9. Phosphorylation of Ser10 interferes with antibody binding to dimethyl Lys9.

Package Size :

100 µg

Uses :

Western Blotting;ELISA;Immunocytochemistry;Immunoprecipitation;Multiplexing;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.