Sirt1 Antibody, clone 3H10.2 | EMD Millipore 05-1243 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Sirt1 Antibody, clone 3H10.2

catalog :

05-1243

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3H10.2

reactivity :

human

application :

western blot, immunoprecipitation

citations: 1

Reference
Shimada T, Furuta H, Doi A, Ariyasu H, Kawashima H, Wakasaki H, et al. Des-acyl ghrelin protects microvascular endothelial cells from oxidative stress-induced apoptosis through sirtuin 1 signaling pathway. Metabolism. 2014;63:469-74 pubmed publisher

product information

Catalog Number :

05-1243

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Sirt1 Antibody, clone 3H10.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q96EB6

Host Name :

Mouse

Antigen :

Sirt1

Clone :

3H10.2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Sirt1 Antibody, clone 3H10.2

Cross Reactivity :

Human

Background :

The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. There are now five described human homologs of yeast Sir2, which are named SIRT1-5. SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. SIRT1 is a HDAC which is important in establishing repressive chromatin structures and plays a role in increasing lifespan.

Immunogen :

GST fusion protein corresponding to amino acids 508-616 of human Sirt1.

Specificity :

Human Sirt1. Does not cross react with lysates from mouse NIH/3T3 cells or rat PC12 cells.

Package Size :

100 μg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Maintain at 2-8 °C in undiluted aliquots for up to 6 months after date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.