trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4 | EMD Millipore 05-1242 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4

catalog :

05-1242

quantity :

200 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F12-H4

reactivity :

human, mouse, fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; loading ...; fig 2s1c	Del Rosario B, Kriz A, Del Rosario A, Anselmo A, Fry C, White F, et al. Exploration of CTCF post-translation modifications uncovers Serine-224 phosphorylation by PLK1 at pericentric regions during the G2/M transition. elife. 2019;8: pubmed publisher
western blot; human; fig s3	Renaud E, Barascu A, Rosselli F. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res. 2016;44:648-56 pubmed publisher
chromatin immunoprecipitation; human; 10 ul/mg; fig s5	Su S, Zhao Q, He C, Huang D, Liu J, Chen F, et al. miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program. Nat Commun. 2015;6:8523 pubmed publisher
chromatin immunoprecipitation; human; 1:4 immunocytochemistry; human; 1:200 western blot; human; 1:4000	Santos G, da Silva A, Feldman L, Ventura G, Vassetzky Y, de Moura Gallo C. Epigenetic modifications, chromatin distribution and TP53 transcription in a model of breast cancer progression. J Cell Biochem. 2015;116:533-41 pubmed publisher
chromatin immunoprecipitation; human; fig 4	Zhu X, Liu J, Xu X, Zhang C, Dai D. The Pleckstrin and Sec7 domain-containing gene as a novel epigenetic modification marker in human gastric cancer and its clinical significance. Int J Oncol. 2015;46:195-204 pubmed publisher
immunocytochemistry; mouse; 1:300	Ma P, Pan H, Montgomery R, Olson E, Schultz R. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 2012;109:E481-9 pubmed publisher
western blot; fruit fly ; 1:5000	Egelhofer T, Minoda A, Klugman S, Lee K, Kolasinska Zwierz P, Alekseyenko A, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18:91-3 pubmed publisher
	Chiang M, Michieletto D, Brackley C, Rattanavirotkul N, Mohammed H, Marenduzzo D, et al. Polymer Modeling Predicts Chromosome Reorganization in Senescence. Cell Rep. 2019;28:3212-3223.e6 pubmed publisher
	Wang X, Cao W, Zhang J, Yan M, Xu Q, Wu X, et al. A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination. EMBO J. 2017;36:1243-1260 pubmed publisher
	Chronis C, Fiziev P, Papp B, Butz S, Bonora G, Sabri S, et al. Cooperative Binding of Transcription Factors Orchestrates Reprogramming. Cell. 2017;168:442-459.e20 pubmed publisher
	Teng F, Guo M, Liu F, Wang C, Dong J, Zhang L, et al. Treatment with an SLC12A1 antagonist inhibits tumorigenesis in a subset of hepatocellular carcinomas. Oncotarget. 2016;7:53571-53582 pubmed publisher
	Brideau N, Coker H, Gendrel A, Siebert C, Bezstarosti K, Demmers J, et al. Independent Mechanisms Target SMCHD1 to Trimethylated Histone H3 Lysine 9-Modified Chromatin and the Inactive X Chromosome. Mol Cell Biol. 2015;35:4053-68 pubmed publisher
	Rao M, Matsumoto Y, Richardson M, Panneerdoss S, Bhardwaj A, Ward J, et al. Hormone-induced and DNA demethylation-induced relief of a tissue-specific and developmentally regulated block in transcriptional elongation. J Biol Chem. 2014;289:35087-101 pubmed publisher
	Tang M, Li Y, Zhang X, Deng T, Zhou Z, Ma W, et al. Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) promotes non-homologous end joining and inhibits homologous recombination repair upon DNA damage. J Biol Chem. 2014;289:34024-32 pubmed publisher
	Calderon M, Verway M, Benslama R, Birlea M, Bouttier M, Dimitrov V, et al. Ligand-dependent corepressor contributes to transcriptional repression by C2H2 zinc-finger transcription factor ZBRK1 through association with KRAB-associated protein-1. Nucleic Acids Res. 2014;42:7012-27 pubmed publisher
	Zhang Z, Liu Y, Li S, Guo L, Zhao Y, Qian S, et al. Suv39h1 mediates AP-2?-dependent inhibition of C/EBP? expression during adipogenesis. Mol Cell Biol. 2014;34:2330-8 pubmed publisher
	Zhu X, Liu J, Xu X, Zhang C, Dai D. Novel tumor-suppressor gene epidermal growth factor-containing fibulin-like extracellular matrix protein 1 is epigenetically silenced and associated with invasion and metastasis in human gastric cancer. Mol Med Rep. 2014;9:2283-92 pubmed publisher
	Qi C, Liu S, Qin R, Zhang Y, Wang G, Shang Y, et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci. 2014;34:4494-508 pubmed publisher
	Zhang Q, Lei X, Lu H. Alterations of epigenetic signatures in hepatocyte nuclear factor 4? deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays. PLoS ONE. 2014;9:e84925 pubmed publisher
	Khanal P, Kim G, Lim S, Yun H, Lee K, Choi H, et al. Prolyl isomerase Pin1 negatively regulates the stability of SUV39H1 to promote tumorigenesis in breast cancer. FASEB J. 2013;27:4606-18 pubmed publisher
	Zhang Y, Yang X, Gui B, Xie G, Zhang D, Shang Y, et al. Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27. J Biol Chem. 2011;286:42414-25 pubmed publisher
	Suter M, Bocock P, Showalter L, Hu M, Shope C, McKnight R, et al. Epigenomics: maternal high-fat diet exposure in utero disrupts peripheral circadian gene expression in nonhuman primates. FASEB J. 2011;25:714-26 pubmed publisher
	Gencheva M, Chen C, Nguyen T, Shively J. Regulation of CEACAM1 transcription in human breast epithelial cells. BMC Mol Biol. 2010;11:79 pubmed publisher

product information

Catalog Number :

05-1242

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

trimethyl-Histone H3 (Lys9)

Clone :

6F12-H4

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4

Cross Reactivity :

Human;Mouse

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely via acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α/β. The enzymes responsible for H3K9me3 formation are SUV39H1 and SUV39H2.

ALT Names :

H3K9me3;Histone H3 (tri methyl K9);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated, branched synthetic peptide containing the sequence (QTARme3K- STGGKA)2-KC, in which me3K corresponds to trimethyl lysine 9 of human histone H3. Data suggests this antibody may require the presence of the glutamine residue in position 5 for binding.

Specificity :

Recognizes trimethyl-histone H3 (Lys9), Mr 17 kDa. Cross-reacts with dimethyl histone H3(Lys9) at higher concentrations.

Package Size :

200 µL

Uses :

Peptide Inhibition Assay;Immunofluorescence;Dot Blot;ChIP-seq;Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid freezing, which can damage IgG and affect product performance.