trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4, Trial Size 05-1242-S | EMD Millipore 05-1242-S product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4, Trial Size 05-1242-S

catalog :

05-1242-S

quantity :

20 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F12-H4

reactivity :

human

application :

western blot, immunocytochemistry, chromatin immunoprecipitation

citations: 1

Reference
Chiang M, Michieletto D, Brackley C, Rattanavirotkul N, Mohammed H, Marenduzzo D, et al. Polymer Modeling Predicts Chromosome Reorganization in Senescence. Cell Rep. 2019;28:3212-3223.e6 pubmed publisher

product information

Catalog Number :

05-1242-S

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4, Trial Size 05-1242-S

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Histone H3

Clone :

6F12-H4

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4, Trial Size 05-1242-S

Cross Reactivity :

Human, Mouse

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely via acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α/β. The enzymes responsible for H3K9me3 formation are SUV39H1 and SUV39H2.

ALT Names :

H3K9me3, Histone H3 (tri methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

Immunogen :

KLH-conjugated, branched synthetic peptide containing the sequence (QTARme3K- STGGKA)2-KC, in which me3K corresponds to trimethyl lysine 9 of human histone H3. Data suggests this antibody may require the presence of the glutamine residue in position 5 for binding.

Package Size :

20 µL

Uses :

Chromatin Immunoprecipitation (ChIP), Peptide Inhibition Assay, Immunofluorescence, Dot Blot, ChIP-seq, Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt.For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid freezing, which can damage IgG and affect product performance.