FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 | EMD Millipore 05-117 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

catalog :

05-117

quantity :

500 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

bFM-1

reactivity :

human, mouse, rat, bovine

application :

neutralization, immunoprecipitation, radioimmunoassay, blocking or activating experiments

citations: 21

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; fig 6	Zhao C, Zhang W, Zhao Y, Yang Y, Luo H, Ji G, et al. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism. Sci Rep. 2016;6:19404 pubmed publisher
blocking or activating experiments; human; 5 ug/ml	Azuma K, Kawahara A, Sonoda K, Nakashima K, Tashiro K, Watari K, et al. FGFR1 activation is an escape mechanism in human lung cancer cells resistant to afatinib, a pan-EGFR family kinase inhibitor. Oncotarget. 2014;5:5908-19 pubmed
blocking or activating experiments; human blocking or activating experiments; mouse; 1:200	Santiago J, McNaughton L, Koleini N, Ma X, Bestvater B, Nickel B, et al. High molecular weight fibroblast growth factor-2 in the human heart is a potential target for prevention of cardiac remodeling. PLoS ONE. 2014;9:e97281 pubmed publisher
blocking or activating experiments; rat	Higginson J, Thompson S, Santos Silva A, Guimond S, Turnbull J, Barnett S. Differential sulfation remodelling of heparan sulfate by extracellular 6-O-sulfatases regulates fibroblast growth factor-induced boundary formation by glial cells: implications for glial cell transplantation. J Neurosci. 2012;32:15902-12 pubmed publisher
	Fessler E, Borovski T, Medema J. Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF. Mol Cancer. 2015;14:157 pubmed publisher
	Guerrouahen B, Pasquier J, Kaoud N, Maleki M, Beauchamp M, Yasmeen A, et al. Akt-activated endothelium constitutes the niche for residual disease and resistance to bevacizumab in ovarian cancer. Mol Cancer Ther. 2014;13:3123-36 pubmed publisher
	Traer E, Javidi Sharifi N, Agarwal A, Dunlap J, English I, Martinez J, et al. Ponatinib overcomes FGF2-mediated resistance in CML patients without kinase domain mutations. Blood. 2014;123:1516-24 pubmed publisher
	Ladewig J, Koch P, Brüstle O. Auto-attraction of neural precursors and their neuronal progeny impairs neuronal migration. Nat Neurosci. 2014;17:24-6 pubmed publisher
	Knelson E, Gaviglio A, Tewari A, Armstrong M, Mythreye K, Blobe G. Type III TGF-β receptor promotes FGF2-mediated neuronal differentiation in neuroblastoma. J Clin Invest. 2013;123:4786-98 pubmed
	Sáinz Jaspeado M, Huertas Martínez J, Lagares Tena L, Martín Liberal J, Mateo Lozano S, de Alava E, et al. EphA2-induced angiogenesis in ewing sarcoma cells works through bFGF production and is dependent on caveolin-1. PLoS ONE. 2013;8:e71449 pubmed publisher
	Chang Y, Lin L, Lou C, Chou C, Ch ang H. Bone marrow transplantation rescues intestinal mucosa after whole body radiation via paracrine mechanisms. Radiother Oncol. 2012;105:371-7 pubmed publisher
	Fan Y, Lv Z, Yao X, Wang W, Huang K, Meng Q, et al. Interleukin-25 promotes basic fibroblast growth factor expression by human endothelial cells through interaction with IL-17RB, but not IL-17RA. Clin Exp Allergy. 2012;42:1604-14 pubmed publisher
	Voulgari Kokota A, Fairless R, Karamita M, Kyrargyri V, Tseveleki V, Evangelidou M, et al. Mesenchymal stem cells protect CNS neurons against glutamate excitotoxicity by inhibiting glutamate receptor expression and function. Exp Neurol. 2012;236:161-70 pubmed publisher
	Wang L, Park H, Chhim S, Ding Y, Jiang W, Queen C, et al. A novel monoclonal antibody to fibroblast growth factor 2 effectively inhibits growth of hepatocellular carcinoma xenografts. Mol Cancer Ther. 2012;11:864-72 pubmed publisher
	Shiota M, Hikita Y, Kawamoto Y, Kusakabe H, Tanaka M, Izumi Y, et al. Pravastatin-induced proangiogenic effects depend upon extracellular FGF-2. J Cell Mol Med. 2012;16:2001-9 pubmed publisher
	Santiago J, Ma X, McNaughton L, Nickel B, Bestvater B, Yu L, et al. Preferential accumulation and export of high molecular weight FGF-2 by rat cardiac non-myocytes. Cardiovasc Res. 2011;89:139-47 pubmed publisher
	Gutierrez J, Brandan E. A novel mechanism of sequestering fibroblast growth factor 2 by glypican in lipid rafts, allowing skeletal muscle differentiation. Mol Cell Biol. 2010;30:1634-49 pubmed publisher
	Einstein O, Friedman Levi Y, Grigoriadis N, Ben Hur T. Transplanted neural precursors enhance host brain-derived myelin regeneration. J Neurosci. 2009;29:15694-702 pubmed publisher
	Lefevre G, Babchia N, Calipel A, Mouriaux F, Faussat A, Mrzyk S, et al. Activation of the FGF2/FGFR1 autocrine loop for cell proliferation and survival in uveal melanoma cells. Invest Ophthalmol Vis Sci. 2009;50:1047-57 pubmed publisher
	Begum S, Zhang Y, Shintani T, Toratani S, Sato J, Okamoto T. Immunohistochemical expression of heparin-binding protein 17/fibroblast growth factor-binding protein-1 (HBp17/FGFBP-1) as an angiogenic factor in head and neck tumorigenesis. Oncol Rep. 2007;17:591-6 pubmed
	Watanabe Y, Matsumoto N, Dezawa M, Itokazu Y, Yoshihara T, Ide C. Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite outgrowth and survival of hippocampal neurons. Neurosci Lett. 2005;379:158-63 pubmed

product information

Catalog Number :

05-117

Subcategory :

Cell Signaling

Product Name :

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P09038

Host Name :

Mouse

Antigen :

FGF-2/basic FGF (neutralizing)

Clone :

bFM-1

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

Cross Reactivity :

Bovine;Human;Mouse;Rat

Background :

Basic fibroblast growth factor, also known as bFGF or FGF2, is a member of the fibroblast growth factor family. In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide. It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis. Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined.

ALT Names :

Basic fibroblast growth factor;basic fibroblast growth factor bFGF;fibroblast growth factor 2;fibroblast growth factor 2 (basic);heparin-binding growth factor 2.

Immunogen :

Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.

Specificity :

This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.

Package Size :

500 µg

Uses :

Neutralizing;Radioimmunoassay

Storage :

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.