Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

antibody

JAK1 Antibody, clone 73

05-1154

100 μg

monoclonal

mouse

nonconjugated

73

The same clone is also sold as:

• Santa Cruz Biotechnology: sc-136225

human, mouse, dogs, chicken

western blot, immunocytochemistry, immunoprecipitation

Cell Signaling

Anti-JAK1 Antibody, clone 73

Antibodies

Monoclonal Antibody

P23458

Mouse

JAK1

73

Purified

IgG2b

Anti-JAK1 Antibody, clone 73

Human;Mouse;Rat;Canine;Xenopus;Chicken

JAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. They regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs (Signal Transducers and Activators of Transcription), which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal STAT transcription factors, which subsequently dimerize and translocate to the nucleus where they bind DNA. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 (Tyr1007/1008) are sites of trans- or autophosphorylation in vivo and in vitro kinase reactions. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. JAK3 is required for signaling through cytokine receptors which share a common gamma-chain.

Janus kinase 1;Janus kinase 1 (a protein tyrosine kinase) 1;Tyrosine-protein kinase JAK1

Generated from human JAK1, amino acids 551-776.

Recognizes JAK1 (Janus Kinase 1). The members of the JAK family of cytoplasmic protein tyrosine kinases physically associate with ligand-bound receptors. It is this association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members.

100 μg

Western Blotting;Immunoprecipitation;Immunofluorescence

Stable frozen at -20°C in undiluted aliquots for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.