phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18 | EMD Millipore 05-1140 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18

catalog :

05-1140

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry - free floating section

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; mouse; 1:500 western blot; mouse; 1:500	Philippou A, Minozzo F, Spinazzola J, Smith L, Lei H, Rassier D, et al. Masticatory muscles of mouse do not undergo atrophy in space. FASEB J. 2015;29:2769-79 pubmed publisher
western blot; mouse; 1:500; fig 2	Moorwood C, Philippou A, Spinazzola J, Keyser B, Macarak E, Barton E. Absence of ?-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle. Skelet Muscle. 2014;4:13 pubmed publisher
	Cheng Z, Alba Perez A, González García C, Donnelly H, Llopis Hernández V, Jayawarna V, et al. Nanoscale Coatings for Ultralow Dose BMP-2-Driven Regeneration of Critical-Sized Bone Defects. Adv Sci (Weinh). 2019;6:1800361 pubmed publisher

product information

Catalog Number :

05-1140

Subcategory :

Cell Structure

Product Name :

Anti-phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q05397

Host Name :

Mouse

Antigen :

phospho-Focal Adhesion Kinase (Tyr397)

Clone :

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18

Cross Reactivity :

Human;Mouse

Background :

FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. Tyrosine 397 is the autophosphorylation site of Focal Adhesion Kinase. The site binds Src family SH2 domains and the p85 subunit of PI3-Kinase.

ALT Names :

FADK 1;PTK2 protein tyrosine kinase 2;Protein-tyrosine kinase 2;focal adhesion kinase 1

Immunogen :

Generated from human FAK, a.a. 393-404, phosphorylated on Tyr397.

Specificity :

Reacts specifically with Focal Adhesion Kinase (FAK) when phosphorylated on Tyr397. FAK is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the "Focal Adhesion Targeting" sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation at Tyr-397, and activation of FAK through phosphorylation of Tyr residues (Tyr-576 and Tyr577) in the kinase domain activation loop.

Package Size :

100 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.