erbB-2 Antibody, clone N3/D10 (Cytoplasmic Domain) | EMD Millipore 05-1130 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

erbB-2 Antibody, clone N3/D10 (Cytoplasmic Domain)

catalog :

05-1130

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N3/D10

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:1000; fig 4	Koos B, Cane G, Grannas K, LÃ¶f L, ArngÃ¥rden L, Heldin J, et al. Proximity-dependent initiation of hybridization chain reaction. Nat Commun. 2015;6:7294 pubmed publisher

product information

Catalog Number :

05-1130

Subcategory :

Cell Signaling

Product Name :

Anti-erbB-2 Antibody, clone N3/D10 (Cytoplasmic Domain)

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P04626

Host Name :

Mouse

Antigen :

erbB-2

Clone :

N3/D10

Conjugate :

Affinity Purified

Isotype :

IgG1

Product Description :

Anti-erbB-2 Antibody, clone N3/D10 (Cytoplasmic Domain)

Cross Reactivity :

Human;Mouse;Rat

Background :

v-ErbB was identified as the viral counterpart of the EGF Receptor, and its relatives have adopted this nomenclature (or the HER nomenclature for human proteins). These proteins are activated by Heregulin, Neuregulin and Amphiregulin. ErbB-2/HER-2, also known as neu, is amplified in large numbers of highly aggressive breast cancers, but all the family members are capable of heterodimerization, and together activate downstream signaling pathways. ErbB-3 has a natural "substitution" at the codon which is an invariant Lys residue at the ATP-binding site of all protein kinases. It is therefore inactive as a kinase, and can only become phosphorylated by heterodimerization with another erbB-family member. All members of this family, when Tyr-phosphorylated, can nucleate the formation of signaling complexes through adapter proteins.

ALT Names :

Tyrosine kinase-type cell surface receptor HER2

Immunogen :

Synthetic peptide: Cys-Lys-Gly-Thr-Pro-Thr-Ala-Glu-Asp-Pro-Glu-Tyr-Leu-Gly-Leu-Asp-Val-Pro-Val (amino acids 1238-1255) selected from the cytoplasmic domain of human c-erbB-2 protein.

Specificity :

The antibody reacts with c-erbB-2 which is a membrane associated oncoprotein that has considerable sequence homology with EGFR. Membrane preparations were made from SK-BR-3 mammary adenocarcinoma and A431 epidermoid carcinoma cell lines. SK-BR-3 expresses high levels of c-erbB-2 whilst A431 expresses high levels of EGFR. The antibody has been used to detect c-erbB-2 in paraffin-embedded material from breast tumours.

Package Size :

100 µg

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry (Paraffin);Flow Cytometry

Storage :

For use within 1 month of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.