phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2 | EMD Millipore 05-1087 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2

catalog :

05-1087

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

24.6.2

reactivity :

hamsters, human, mouse, rat, dogs, bovine

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:2000; loading ...; fig 2c	Yan H, Gao Y, Zhang Y. Inhibition of JNK suppresses autophagy and attenuates insulin resistance in a rat model of nonalcoholic fatty liver disease. Mol Med Rep. 2017;15:180-186 pubmed publisher
western blot; hamsters	Hancer N, Qiu W, Cherella C, Li Y, COPPS K, White M. Insulin and metabolic stress stimulate multisite serine/threonine phosphorylation of insulin receptor substrate 1 and inhibit tyrosine phosphorylation. J Biol Chem. 2014;289:12467-84 pubmed publisher

product information

Catalog Number :

05-1087

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35568

Host Name :

Mouse

Antigen :

phospho-IRS1 (Ser307 mouse/ Ser312 human)

Clone :

24.6.2

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2

Cross Reactivity :

Human;Mouse;Rat;Monkey;Bovine;Canine;Pig

Background :

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

ALT Names :

insulin receptor substrate 1

Immunogen :

Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser307 of mouse IRS1.

Specificity :

This antibody recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human).

Package Size :

100 µg

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry;Immunofluorescence

Storage :

Stable for 1 year at 2-8ºC from date of receipt.