IRS1 Antibody, clone 4.2.2 | EMD Millipore 05-1085 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

IRS1 Antibody, clone 4.2.2

catalog :

05-1085

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4.2.2

reactivity :

hamsters, human, mouse, dogs, bovine, pigs

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; mouse; loading ...; fig 1c	Araiz C, Yan A, Bettedi L, Samuelson I, Virtue S, McGavigan A, et al. Enhanced β-adrenergic signalling underlies an age-dependent beneficial metabolic effect of PI3K p110α inactivation in adipose tissue. Nat Commun. 2019;10:1546 pubmed publisher
western blot; hamsters	Hancer N, Qiu W, Cherella C, Li Y, COPPS K, White M. Insulin and metabolic stress stimulate multisite serine/threonine phosphorylation of insulin receptor substrate 1 and inhibit tyrosine phosphorylation. J Biol Chem. 2014;289:12467-84 pubmed publisher
western blot; pigs	Guo Y, Chen Y, Zhang Y, Zhang Y, Chen L, Mo D. Up-regulated miR-145 expression inhibits porcine preadipocytes differentiation by targeting IRS1. Int J Biol Sci. 2012;8:1408-17 pubmed publisher
	Li J, Xu L, Dong H, Huang Z, Zhao Y, Chen G, et al. Effects of Chinese Fructus Mume formula and its separated prescription extract on insulin resistance in type 2 diabetic rats. J Huazhong Univ Sci Technolog Med Sci. 2013;33:877-885 pubmed publisher
	Ye L, Varamini B, Lamming D, Sabatini D, Baur J. Rapamycin has a biphasic effect on insulin sensitivity in C2C12 myotubes due to sequential disruption of mTORC1 and mTORC2. Front Genet. 2012;3:177 pubmed publisher

product information

Catalog Number :

05-1085

Subcategory :

Cell Signaling

Product Name :

Anti-IRS1 Antibody, clone 4.2.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35568

Host Name :

Mouse

Antigen :

IRS1

Clone :

4.2.2

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-IRS1 Antibody, clone 4.2.2

Cross Reactivity :

Human;Mouse;Rat;Monkey;Bovine;Canine;Pig

Background :

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

ALT Names :

insulin receptor substrate 1

Immunogen :

Synthetic peptide corresponding to amino acids 431-446 of mouse IRS1.

Specificity :

This antibody recognizes IRS1.

Package Size :

100 µg

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry

Storage :

Stable for 1 year at 2-8ºC from date of receipt.