Ras Antibody, (K-, H-, N-), clone 9A11.2 | EMD Millipore 05-1072 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Ras Antibody, (K-, H-, N-), clone 9A11.2

catalog :

05-1072

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9A11.2

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2e	Xiong X, Lee C, Li W, Yu J, Zhu L, Kim Y, et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open. 2019;: pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig s2 western blot; human; fig 1b	Waters A, Ozkan Dagliyan I, Vaseva A, Fer N, Strathern L, Hobbs G, et al. Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. Sci Signal. 2017;10: pubmed publisher
	Wang Z, Feng Y, Bardeesy N, Bardessy N, WONG K, Liu X, et al. Temporal dissection of K-ras(G12D) mutant in vitro and in vivo using a regulatable K-ras(G12D) mouse allele. PLoS ONE. 2012;7:e37308 pubmed publisher
	Yang Y, Tao Cheng J, Reese T, Dosemeci A. SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience. 2011;192:132-9 pubmed publisher

product information

Catalog Number :

05-1072

Subcategory :

Cell Signaling

Product Name :

Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P01116

Host Name :

Mouse

Antigen :

Ras

Clone :

9A11.2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2

Cross Reactivity :

Human;Mouse;Rat

Background :

Ras, a proto-oncogene, is a small G-protein that has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in there approximately 20 C-terminal amino acids. H-Ras was first discovered as a transforming product the retrovirus Harvey murine virus and K-Ras of Kirten sarcoma virus. Ras is a heavily studied target of both academic and pharmaceutical research because of its implications in various pathways and diseases as well as being mutated in a large number of human cancers. Ras is most notably the activator of the Erk/MAPK kinase pathway as activator of Raf, as well as an activator of PI3 Kinase (PI3K). In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways including the MAPK pathway through its activation of Raf, but also others as well that include PI3 Kinase and RalGDS. One path that the pharmaceutical industry has taken to control Ras and its activity is by finding what some consider its Achilles’ heel. For its activation, Ras must localize to the plasma membrane, but interestingly, it lacks a transmembrane domain. To achieve this, Ras must first undergo a post-translational modification (PTM) known as prenylation or geranylation at its C-terminal CAAX motif. For this to take place, a controlled three step process must occur. The first step in the process is the prenylation or geranylation of the C in the CAAX motif that is initiated by the covalent attachment of farnesyl groups to the cysteine that is catalyzed by the heterodimer enzymes farnesyl transferases and . After this modification, the –aaX of the motif is proteolytically removed via Rce1 (Ras Converting Enzyme 1), a membrane associated endoprotease, by a mechanism that is still not fully understood. Finally, the C-terminal prenylcysteine is now methlylated by ICMT (Isoprenylcysteine Carboxymethyl Transferase). These drugs have yet to pass clinical trials though and there is doubt that they will ever be successful in treating tumors associated with Ras activation.

Immunogen :

Full length recombinant GST-tagged human H-Ras.

Specificity :

Recognizes K-, H-, and N-Ras (all 3 isofroms).

Package Size :

100 μg

Uses :

Western Blotting;Immunohistochemistry (Paraffin)

Storage :

Stable for 1 year at 4°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.