phospho-PRAS40 (Thr246) Antibody, clone 4H2.2 | EMD Millipore 05-1069 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-PRAS40 (Thr246) Antibody, clone 4H2.2

catalog :

05-1069

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4H2.2

reactivity :

human, mouse, bovine

application :

western blot, immunocytochemistry, immunoprecipitation

product information

Catalog Number :

05-1069

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q96B36

Host Name :

Mouse

Antigen :

phospho-PRAS40 (Thr246)

Clone :

4H2.2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2

Cross Reactivity :

Human;Mouse

Background :

PRAS40 (Proline Rich Akt Substrate, 40kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1. It is thought that through its binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.

ALT Names :

40 kDa proline-rich AKT substrate AKT1 substrate 1 (proline-rich) proline-rich Akt substrate, 40 kDa

Immunogen :

Synthetic peptide corresponding to the amino acids surrounding and including phosphorylated Thr246 of human PRAS40.

Specificity :

Detects PRAS40 when phosphorylated on Thr246.

Package Size :

100 µg

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry

Storage :

Stable for 1 year at 2-8ºC from date of receipt.