Phosphotyrosine Antibody, 4G10® Platinum | EMD Millipore 05-1050X product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Phosphotyrosine Antibody, 4G10® Platinum

catalog :

05-1050X

quantity :

30 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G10

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 84

Reference
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product information

Catalog Number :

05-1050X

Subcategory :

Cell Signaling

Product Name :

Anti-Phosphotyrosine Antibody, 4G10® Platinum

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

Phosphotyrosine

Clone :

4G10

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-Phosphotyrosine Antibody, 4G10® Platinum

Cross Reactivity :

All

Background :

The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.

Immunogen :

4G10 ® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein.

Specificity :

Tyrosine-phosphorylated proteins from all species

Package Size :

30 μL

Uses :

Western Blotting;Immunofluorescence;Flow Cytometry;Immunohistochemistry;Immunoprecipitation;Immunocytochemistry;ELISA

Storage :

2 years at 2-8°C from date of shipment. NOTE: DO NOT FREEZE. For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.