This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
catalog :
05-1050
quantity :
100 µL
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 29
Reference
Thomson B, Carota I, Souma T, Soman S, Vestweber D, Quaggin S. Targeting the vascular-specific phosphatase PTPRB protects against retinal ganglion cell loss in a pre-clinical model of glaucoma. elife. 2019;8: pubmed publisher
Böhler P, Stuhldreier F, Anand R, Kondadi A, Schlütermann D, Berleth N, et al. The mycotoxin phomoxanthone A disturbs the form and function of the inner mitochondrial membrane. Cell Death Dis. 2018;9:286 pubmed publisher
Xian X, Ding Y, Dieckmann M, Zhou L, Plattner F, Liu M, et al. LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis. elife. 2017;6: pubmed publisher
Tsutsumi R, Harizanova J, Stockert R, Schröder K, Bastiaens P, Neel B. Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2. Nat Commun. 2017;8:466 pubmed publisher
Bender K, Blackburn R, Monaghan J, Derbyshire P, Menke F, Zipfel C, et al. Autophosphorylation-based Calcium (Ca2+) Sensitivity Priming and Ca2+/Calmodulin Inhibition of Arabidopsis thaliana Ca2+-dependent Protein Kinase 28 (CPK28). J Biol Chem. 2017;292:3988-4002 pubmed publisher
GALLEGOS L, Ng M, Sowa M, Selfors L, White A, Zervantonakis I, et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for β-catenin. Sci Rep. 2016;6:27114 pubmed publisher
Kii I, Sumida Y, Goto T, Sonamoto R, Okuno Y, Yoshida S, et al. Selective inhibition of the kinase DYRK1A by targeting its folding process. Nat Commun. 2016;7:11391 pubmed publisher
Nakazawa T, Hashimoto R, Sakoori K, Sugaya Y, Tanimura A, Hashimotodani Y, et al. Emerging roles of ARHGAP33 in intracellular trafficking of TrkB and pathophysiology of neuropsychiatric disorders. Nat Commun. 2016;7:10594 pubmed publisher
Luberg K, Park R, Aleksejeva E, Timmusk T. Novel transcripts reveal a complex structure of the human TRKA gene and imply the presence of multiple protein isoforms. BMC Neurosci. 2015;16:78 pubmed publisher
Bollu L, Ren J, Blessing A, Katreddy R, Gao G, Xu L, et al. Involvement of de novo synthesized palmitate and mitochondrial EGFR in EGF induced mitochondrial fusion of cancer cells. Cell Cycle. 2014;13:2415-30 pubmed publisher
Edelmann M, Shack L, Naske C, Walters K, Nanduri B. SILAC-based quantitative proteomic analysis of human lung cell response to copper oxide nanoparticles. PLoS ONE. 2014;9:e114390 pubmed publisher
Keegan K, Li C, Li Z, Ma J, Ragains M, Coberly S, et al. Preclinical evaluation of AMG 925, a FLT3/CDK4 dual kinase inhibitor for treating acute myeloid leukemia. Mol Cancer Ther. 2014;13:880-9 pubmed publisher
Basu S, Rajakaruna S, De Arcangelis A, Zhang L, Georges Labouesse E, Menko A. ?6 integrin transactivates insulin-like growth factor receptor-1 (IGF-1R) to regulate caspase-3-mediated lens epithelial cell differentiation initiation. J Biol Chem. 2014;289:3842-55 pubmed publisher
Shrivastava V, Marmor H, Chernyak S, Goldstein M, Feliciano M, Vigodner M. Cigarette smoke affects posttranslational modifications and inhibits capacitation-induced changes in human sperm proteins. Reprod Toxicol. 2014;43:125-9 pubmed publisher
Olavarría V, Figueroa J, Mulero V. Induction of genes encoding NADPH oxidase components and activation of IFN regulatory factor-1 by prolactin in fish macrophages. Innate Immun. 2013;19:644-54 pubmed publisher
Ma Y, Lovekamp Swan T, Bekele W, Dohi A, Schreihofer D. Hypoxia-inducible factor and vascular endothelial growth factor are targets of dietary soy during acute stroke in female rats. Endocrinology. 2013;154:1589-97 pubmed publisher
Meyer R, D Alessandro L, Kar S, Kramer B, She B, Kaschek D, et al. Heterogeneous kinetics of AKT signaling in individual cells are accounted for by variable protein concentration. Front Physiol. 2012;3:451 pubmed publisher
Vigodner M, Shrivastava V, Gutstein L, Schneider J, Nieves E, Goldstein M, et al. Localization and identification of sumoylated proteins in human sperm: excessive sumoylation is a marker of defective spermatozoa. Hum Reprod. 2013;28:210-23 pubmed publisher
Linger R, Cohen R, Cummings C, Sather S, Migdall Wilson J, Middleton D, et al. Mer or Axl receptor tyrosine kinase inhibition promotes apoptosis, blocks growth and enhances chemosensitivity of human non-small cell lung cancer. Oncogene. 2013;32:3420-31 pubmed publisher
Sarkar T, Sharan S, Wang J, Pawar S, Cantwell C, Johnson P, et al. Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells. Mol Cell Biol. 2012;32:320-32 pubmed publisher
Olavarría V, Figueroa J, Mulero V. Prolactin-induced activation of phagocyte NADPH oxidase in the teleost fish gilthead seabream involves the phosphorylation of p47phox by protein kinase C. Dev Comp Immunol. 2012;36:216-21 pubmed publisher
Helmer R, Dertien J, Chilton B. Prolactin induces Jak2 phosphorylation of RUSHY195. Mol Cell Endocrinol. 2011;338:79-83 pubmed publisher
Saiya Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92 pubmed publisher
Driessens G, Zheng Y, Locke F, Cannon J, Gounari F, Gajewski T. Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-?1 phosphorylation. J Immunol. 2011;186:784-90 pubmed publisher
Helmer R, Panchoo M, Dertien J, Bhakta S, Hewetson A, Chilton B. Prolactin-induced Jak2 phosphorylation of RUSH: a key element in Jak/RUSH signaling. Mol Cell Endocrinol. 2010;325:143-9 pubmed publisher
Wang H, Wu J, Chia J, Yang C, Wu Y, Juang R. Phytochelatin synthase is regulated by protein phosphorylation at a threonine residue near its catalytic site. J Agric Food Chem. 2009;57:7348-55 pubmed publisher
Marks D, Tucker K, Cavallin M, Mast T, Fadool D. Awake intranasal insulin delivery modifies protein complexes and alters memory, anxiety, and olfactory behaviors. J Neurosci. 2009;29:6734-51 pubmed publisher
Huot M, Brown C, Lamarche Vane N, Richard S. An adaptor role for cytoplasmic Sam68 in modulating Src activity during cell polarization. Mol Cell Biol. 2009;29:1933-43 pubmed publisher
Huang J, Bae J, Tsai J, Kadenhe Chiweshe A, Papa J, Lee A, et al. Angiopoietin-1/Tie-2 activation contributes to vascular survival and tumor growth during VEGF blockade. Int J Oncol. 2009;34:79-87 pubmed
product information
Catalog Number :
05-1050
Subcategory :
Cell Signaling
Product Name :
4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
4G10® Platinum
Conjugate :
Purified
Isotype :
IgG2b
Product Description :
4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
Cross Reactivity :
All
Background :
The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
Immunogen :
4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
Specificity :
Tyrosine-phosphorylated proteins from all species
Package Size :
100 µL
Uses :
Western Blotting;Immunocytochemistry;ELISA;Immunohistochemistry;Immunofluorescence;Flow Cytometry;Immunoprecipitation
Storage :
Stable for 1 year at from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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