Sin1 Antibody, clone 1C7.2 | EMD Millipore 05-1044 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Sin1 Antibody, clone 1C7.2

catalog :

05-1044

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1C7.2

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 5d	Mathieu J, Detraux D, Kuppers D, Wang Y, Cavanaugh C, Sidhu S, et al. Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency. Nat Commun. 2019;10:632 pubmed publisher
western blot; human; loading ...; fig 4b	Ebner M, Sinkovics B, Szczygieł M, Ribeiro D, Yudushkin I. Localization of mTORC2 activity inside cells. J Cell Biol. 2017;216:343-353 pubmed publisher
western blot; human	Verma R, Marchese A. The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR. J Biol Chem. 2015;290:6810-24 pubmed publisher
western blot; rat; 1:1000	Gokhale N, Zaremba A, Janoshazi A, Weaver J, Shears S. PPIP5K1 modulates ligand competition between diphosphoinositol polyphosphates and PtdIns(3,4,5)P3 for polyphosphoinositide-binding domains. Biochem J. 2013;453:413-26 pubmed publisher
western blot; human; 1:1000	Wang Y, Liu J, Liu C, Naji A, Stoffers D. MicroRNA-7 regulates the mTOR pathway and proliferation in adult pancreatic ?-cells. Diabetes. 2013;62:887-95 pubmed publisher
	Sarbassov D, Bulgakova O, Bersimbaev R, Shaiken T. Isolation of the mTOR complexes by affinity purification. Methods Mol Biol. 2012;821:59-74 pubmed publisher

product information

Catalog Number :

05-1044

Subcategory :

Cell Signaling

Product Name :

Anti-Sin1 Antibody, clone 1C7.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9BPZ7

Host Name :

Mouse

Antigen :

Sin1

Clone :

1C7.2

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-Sin1 Antibody, clone 1C7.2

Cross Reactivity :

Human;Mouse;Rat

Background :

Sin1 (stress-activated protein kinase (SAPK)-interacting protein 1, MAPKAP1) is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase along with Sin1, GL (mLST8), Protor1, Protor2, and Rictor. The complex that is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Sin1 is also known to directly interact without proteins that include Ras, SPAKs, and JNK. Sin1 is known to have multiple alternative splicing isoforms. Full-length Sin1 is a 522aa protein (59kDa). Sin1 (Isoform 5) (36 kDa) has exon 6 spliced to an alternative exon 7a that results in the loss of both the RBD and PHL domains. Sin1Isoform 2) (55 kDa) lacks exon 7 and lacks the RBD. Sin1 (Isoform 3) (53 kDa) lacks exon 8 lacks the PHL domain. Sin1 (Isoform 4) (37 kDa) lacks exon 1.

ALT Names :

MEKK2-interacting protein 1;Mitogen-activated protein kinase 2-associated protein 1;SAPK-interacting protein 1 ;Stress-activated map kinase-interacting protein 1 ;TORC2 subunit MAPKAP1;mitogen-activated protein kinase associated protein 1;ras inhibitor MGC2745;stress-activated map kinase interacting protein 1;stress-activated protein kinase-interacting 1

Immunogen :

Full-length Human Sin1 GST-fusion protein.

Specificity :

Detects Sin-1 and its isoforms.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation;Immunohistochemistry (Paraffin);Immunofluorescence

Storage :

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.