EGFR Antibody, clone LA22 | EMD Millipore 05-104 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EGFR Antibody, clone LA22

catalog :

05-104

quantity :

250 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LA22

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 1b	Larsen M, Pérez Verdaguer M, Schmidt B, Bruchez M, Watkins S, Sorkin A. Generation of endogenous pH-sensitive EGF receptor and its application in high-throughput screening for proteins involved in clathrin-mediated endocytosis. elife. 2019;8: pubmed publisher
western blot; human; tbl s3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
western blot; human	De Santis R, Rosi A, Anastasi A, Chiapparino C, Albertoni C, Leoni B, et al. Efficacy of aerosol therapy of lung cancer correlates with EGFR paralysis induced by AvidinOX-anchored biotinylated Cetuximab. Oncotarget. 2014;5:9239-55 pubmed
	Otsuki R, Yamamoto M, Matsumoto E, Iwamoto S, Sezutsu H, Suzui M, et al. Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial. Proc Natl Acad Sci U S A. 2017;114:6740-6745 pubmed publisher
	Pasternack J, Domogauer J, Khullar A, Akudugu J, Howell R. The advantage of antibody cocktails for targeted alpha therapy depends on specific activity. J Nucl Med. 2014;55:2012-9 pubmed publisher
	Yu S, Liu H, Wang S, Chang C, Tsai C, Chao J. Evidence of securin-mediated resistance to gefitinib-induced apoptosis in human cancer cells. Chem Biol Interact. 2013;203:412-22 pubmed publisher
	Rogers K, Kikawa K, Mouradian M, Hernandez K, McKinnon K, Ahwah S, et al. Docosahexaenoic acid alters epidermal growth factor receptor-related signaling by disrupting its lipid raft association. Carcinogenesis. 2010;31:1523-30 pubmed publisher
	Duex J, Sorkin A. RNA interference screen identifies Usp18 as a regulator of epidermal growth factor receptor synthesis. Mol Biol Cell. 2009;20:1833-44 pubmed publisher
	Deshmukh H, Case L, Wesselkamper S, Borchers M, Martin L, Shertzer H, et al. Metalloproteinases mediate mucin 5AC expression by epidermal growth factor receptor activation. Am J Respir Crit Care Med. 2005;171:305-14 pubmed

product information

Catalog Number :

05-104

Subcategory :

Cell Signaling

Product Name :

Anti-EGFR Antibody, clone LA22

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P00533

Host Name :

Mouse

Antigen :

EGFR

Clone :

LA22

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-EGFR Antibody, clone LA22

Cross Reactivity :

Human

Background :

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

ALT Names :

Receptor tyrosine-protein kinase ErbB-1;avian erythroblastic leukemia viral (v-erb-b) oncogene homolog;cell growth inhibiting protein 40;cell proliferation-inducing protein 61;epidermal growth factor receptor;epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog);epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Immunogen :

A431 membrane proteins

Specificity :

Recognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kDa, (based on numbering without the signal peptide) (Wu et al., 1989).

Package Size :

250 μg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.