phospho-Akt (Ser473) Antibody, clone 6F5 | EMD Millipore 05-1003 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Akt (Ser473) Antibody, clone 6F5

catalog :

05-1003

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F5

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 7a	Castella B, Kopecka J, Sciancalepore P, Mandili G, Foglietta M, Mitro N, et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells. Nat Commun. 2017;8:15663 pubmed publisher
western blot; human; 1:500; loading ...; fig s1b	Kuo W, Hwu L, Wu C, Lee J, Chang C, Liu R. STAT3/NF-?B-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer. Theranostics. 2017;7:647-663 pubmed publisher
western blot; mouse; fig 8	Miyamoto Y, Torii T, Takada S, Ohno N, Saitoh Y, Nakamura K, et al. Involvement of the Tyro3 receptor and its intracellular partner Fyn signaling in Schwann cell myelination. Mol Biol Cell. 2015;26:3489-503 pubmed publisher
western blot; human; 1:1000	WILLIAMS K, KILLEBREW D, Clary G, Seawell J, Meeker R. Differential regulation of macrophage phenotype by mature and pro-nerve growth factor. J Neuroimmunol. 2015;285:76-93 pubmed publisher
immunocytochemistry; mouse; fig 5d	Li X, Li J, Wilson A, Sipple J, Schick J, Pang Q. Fancd2 is required for nuclear retention of Foxo3a in hematopoietic stem cell maintenance. J Biol Chem. 2015;290:2715-27 pubmed publisher
western blot; human; 1:2000; tbl 4	Cox S. Intracellular signaling of CTLs. Methods Mol Biol. 2014;1186:49-63 pubmed publisher
	Wang F, Yang L, Shi L, Li Q, Zhang G, Wu J, et al. Nuclear translocation of fibroblast growth factor-2 (FGF2) is regulated by Karyopherin-Î²2 and Ran GTPase in human glioblastoma cells. Oncotarget. 2015;6:21468-78 pubmed

product information

Catalog Number :

05-1003

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-Akt (Ser473) Antibody, clone 6F5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31749

Host Name :

Mouse

Antigen :

phospho-Akt (Ser473)

Clone :

6F5

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-Akt (Ser473) Antibody, clone 6F5

Cross Reactivity :

Human;Mouse;Rat;Vertebrates

Background :

Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including glucose metabolism, cell survival/apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals three isoforms of Akt (Akt1/PKB, Akt2/PKB, and Akt3/PKB)exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module to direct Akt to PIP3 at the cell membrane that is necessary for its activation, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of Akt is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

ALT Names :

Protein kinase B;RAC-alpha serine/threonine-protein kinase;murine thymoma viral (v-akt) oncogene homolog-1;rac protein kinase alpha;v-akt murine thymoma viral oncogene homolog 1

Immunogen :

Peptide encompassing and including phosphorylated Ser473 of human Akt1.

Specificity :

Recognizes Akt only when phosphorylated on Ser473. This antibody does not cross-react with Akt1/PKBα when it is not phosphorylated on Ser473.

Package Size :

100 μg

Uses :

ELISA;Western Blotting;Flow Cytometry;Immunofluorescence;Immunohistochemistry

Storage :

Stable for 1 year at 2-8ºC from date of receipt.