Phosphoserine Antibody, clone 4A4 (mouse IgG1) | EMD Millipore 05-1000 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Phosphoserine Antibody, clone 4A4 (mouse IgG1)

catalog :

05-1000

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4A4

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 3f	Wu Q, Li G, Wen C, Zeng T, Fan Y, Liu C, et al. Monoubiquitination of p120-catenin is essential for TGFβ-induced epithelial-mesenchymal transition and tumor metastasis. Sci Adv. 2020;6:eaay9819 pubmed publisher
western blot; human; loading ...	Brzezinski J, Felkner R, Modi A, Liu M, Roth M. Phosphorylation Requirement of Murine Leukemia Virus p12. J Virol. 2016;90:11208-11219 pubmed
western blot; human; fig 3f	Mir R, Bele A, Mirza S, Srivastava S, Olou A, Ammons S, et al. A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression. Mol Cell Biol. 2015;36:886-99 pubmed publisher
	Hwang C, Wagley Y, Law P, Wei L, Loh H. Phosphorylation of poly(rC) binding protein 1 (PCBP1) contributes to stabilization of mu opioid receptor (MOR) mRNA via interaction with AU-rich element RNA-binding protein 1 (AUF1) and poly A binding protein (PABP). Gene. 2017;598:113-130 pubmed publisher
	Stikvoort A, Sundin M, Uzunel M, Gertow J, Sundberg B, Schaffer M, et al. Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?. PLoS ONE. 2016;11:e0154737 pubmed publisher
	Sadeqzadeh E, de Bock C, O Donnell M, Timofeeva A, Burns G, Thorne R. FAT1 cadherin is multiply phosphorylated on its ectodomain but phosphorylation is not catalysed by the four-jointed homologue. FEBS Lett. 2014;588:3511-7 pubmed publisher
	Oberlander J, Porter D, Onakomaiya M, Penatti C, Vithlani M, Moss S, et al. Estrous cycle variations in GABA(A) receptor phosphorylation enable rapid modulation by anabolic androgenic steroids in the medial preoptic area. Neuroscience. 2012;226:397-410 pubmed publisher
	Lu Q, Wainwright M, Harris V, Aggarwal S, Hou Y, Rau T, et al. Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures. Free Radic Biol Med. 2012;53:1139-51 pubmed publisher
	Velázquez Flores M, Salceda R. Glycine receptor internalization by protein kinases activation. Synapse. 2011;65:1231-8 pubmed publisher

product information

Catalog Number :

05-1000

Subcategory :

Cell Signaling

Product Name :

Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

Phosphoserine

Clone :

4A4

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)

Cross Reactivity :

Vertebrates

Background :

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues ( 95%).

Immunogen :

Phosphoserine coupled to KLH.

Specificity :

This antibody recognizes serine-phosphorylated proteins from all species.

Package Size :

100 µg

Uses :

ELISA;Flow Cytometry;Immunofluorescence;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.