This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
HDAC1 Antibody, clone 2E10
catalog :
05-100
quantity :
100 μL
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2E10
reactivity :
human, mouse
application :
western blot, immunoprecipitation, chromatin immunoprecipitation, western blot knockout validation
citations: 37
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:1000; fig s1
Kwon D, Eom G, Ko J, Shin S, Joung H, Choe N, et al. MDM2 E3 ligase-mediated ubiquitination and degradation of HDAC1 in vascular calcification. Nat Commun. 2016;7:10492 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 1
  • western blot; human; loading ...; fig 1
Citro S, Chiocca S. Assessing the Role of Paralog-Specific Sumoylation of HDAC1. Methods Mol Biol. 2017;1510:329-337 pubmed
  • western blot; mouse; fig 6
Ow J, Palanichamy Kala M, Rao V, Choi M, Bharathy N, Taneja R. G9a inhibits MEF2C activity to control sarcomere assembly. Sci Rep. 2016;6:34163 pubmed publisher
  • western blot; mouse; 1:1000; fig s1
Pinz S, Unser S, Rascle A. Signal transducer and activator of transcription STAT5 is recruited to c-Myc super-enhancer. BMC Mol Biol. 2016;17:10 pubmed publisher
  • western blot; human; loading ...; fig s1
Sakurai T, Isogaya K, Sakai S, Morikawa M, Morishita Y, Ehata S, et al. RNA-binding motif protein 47 inhibits Nrf2 activity to suppress tumor growth in lung adenocarcinoma. Oncogene. 2016;35:5000-9 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 3
Wilson C, Jurk D, Fullard N, Banks P, Page A, Luli S, et al. NFκB1 is a suppressor of neutrophil-driven hepatocellular carcinoma. Nat Commun. 2015;6:6818 pubmed publisher
  • chromatin immunoprecipitation; human
  • western blot; human
Pinz S, Unser S, Buob D, Fischer P, Jobst B, Rascle A. Deacetylase inhibitors repress STAT5-mediated transcription by interfering with bromodomain and extra-terminal (BET) protein function. Nucleic Acids Res. 2015;43:3524-45 pubmed publisher
  • western blot; human; fig 4
Hennig D, Müller S, Wichmann C, Drube S, Pietschmann K, Pelzl L, et al. Antagonism between granulocytic maturation and deacetylase inhibitor-induced apoptosis in acute promyelocytic leukaemia cells. Br J Cancer. 2015;112:329-37 pubmed publisher
  • western blot; human
Isogaya K, Koinuma D, Tsutsumi S, Saito R, Miyazawa K, Aburatani H, et al. A Smad3 and TTF-1/NKX2-1 complex regulates Smad4-independent gene expression. Cell Res. 2014;24:994-1008 pubmed publisher
  • western blot; mouse
Conradt L, Henrich A, Wirth M, Reichert M, Lesina M, Algül H, et al. Mdm2 inhibitors synergize with topoisomerase II inhibitors to induce p53-independent pancreatic cancer cell death. Int J Cancer. 2013;132:2248-57 pubmed publisher
  • chromatin immunoprecipitation; human
  • western blot; human
Karius T, Schnekenburger M, Ghelfi J, Walter J, Dicato M, Diederich M. Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines. Biochem Pharmacol. 2011;81:1329-42 pubmed publisher
  • chromatin immunoprecipitation; human
  • western blot; human
Milagre I, Nunes M, Moutinho M, Rivera I, Fuso A, Scarpa S, et al. Chromatin-modifying agents increase transcription of CYP46A1, a key player in brain cholesterol elimination. J Alzheimers Dis. 2010;22:1209-21 pubmed publisher
Kawasaki N, Isogaya K, Dan S, Yamori T, Takano H, Yao R, et al. TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling. Cell Discov. 2018;4:1 pubmed publisher
Clift D, McEwan W, Labzin L, Konieczny V, Mogessie B, James L, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017;171:1692-1706.e18 pubmed publisher
Gao Y, Westfield G, Erickson J, Cerione R, Skiniotis G, Ramachandran S. Isolation and structure-function characterization of a signaling-active rhodopsin-G protein complex. J Biol Chem. 2017;292:14280-14289 pubmed publisher
Gonzalez Lizarraga F, Socias S, Avila C, Torres Bugeau C, Barbosa L, Binolfi A, et al. Repurposing doxycycline for synucleinopathies: remodelling of α-synuclein oligomers towards non-toxic parallel beta-sheet structured species. Sci Rep. 2017;7:41755 pubmed publisher
Kadiyala V, Patrick N, Mathieu W, Jaime Frias R, Pookhao N, An L, et al. Class I lysine deacetylases facilitate glucocorticoid-induced transcription. J Biol Chem. 2013;288:28900-12 pubmed publisher
Bigley T, Reitsma J, Mirza S, Terhune S. Human cytomegalovirus pUL97 regulates the viral major immediate early promoter by phosphorylation-mediated disruption of histone deacetylase 1 binding. J Virol. 2013;87:7393-408 pubmed publisher
Mounce B, Mboko W, Bigley T, Terhune S, Tarakanova V. A conserved gammaherpesvirus protein kinase targets histone deacetylases 1 and 2 to facilitate viral replication in primary macrophages. J Virol. 2013;87:7314-25 pubmed publisher
Rudra D, deRoos P, Chaudhry A, Niec R, Arvey A, Samstein R, et al. Transcription factor Foxp3 and its protein partners form a complex regulatory network. Nat Immunol. 2012;13:1010-9 pubmed publisher
Gantner B, Jin H, Qian F, Hay N, He B, Ye R. The Akt1 isoform is required for optimal IFN-β transcription through direct phosphorylation of β-catenin. J Immunol. 2012;189:3104-11 pubmed publisher
Parolin C, Calonghi N, Presta E, Boga C, Caruana P, Naldi M, et al. Mechanism and stereoselectivity of HDAC I inhibition by (R)-9-hydroxystearic acid in colon cancer. Biochim Biophys Acta. 2012;1821:1334-40 pubmed publisher
Pacheco M, Nielsen T. Histone deacetylase 1 and 2 in mesenchymal tumors. Mod Pathol. 2012;25:222-30 pubmed publisher
Morikawa M, Koinuma D, Tsutsumi S, Vasilaki E, Kanki Y, Heldin C, et al. ChIP-seq reveals cell type-specific binding patterns of BMP-specific Smads and a novel binding motif. Nucleic Acids Res. 2011;39:8712-27 pubmed publisher
Guo H, Friedman A. Phosphorylation of RUNX1 by cyclin-dependent kinase reduces direct interaction with HDAC1 and HDAC3. J Biol Chem. 2011;286:208-15 pubmed publisher
Waby J, Chirakkal H, Yu C, Griffiths G, Benson R, Bingle C, et al. Sp1 acetylation is associated with loss of DNA binding at promoters associated with cell cycle arrest and cell death in a colon cell line. Mol Cancer. 2010;9:275 pubmed publisher
Godmann M, May E, Kimmins S. Epigenetic mechanisms regulate stem cell expressed genes Pou5f1 and Gfra1 in a male germ cell line. PLoS ONE. 2010;5:e12727 pubmed publisher
Bhandari D, Seo K, Jung J, Kim H, Yang S, Kang K. The regulatory role of c-MYC on HDAC2 and PcG expression in human multipotent stem cells. J Cell Mol Med. 2011;15:1603-14 pubmed publisher
Warrener R, Chia K, Warren W, Brooks K, Gabrielli B. Inhibition of histone deacetylase 3 produces mitotic defects independent of alterations in histone H3 lysine 9 acetylation and methylation. Mol Pharmacol. 2010;78:384-93 pubmed publisher
von Burstin J, Eser S, Paul M, Seidler B, Brandl M, Messer M, et al. E-cadherin regulates metastasis of pancreatic cancer in vivo and is suppressed by a SNAIL/HDAC1/HDAC2 repressor complex. Gastroenterology. 2009;137:361-71, 371.e1-5 pubmed publisher
Kajiwara Y, Akram A, Katsel P, Haroutunian V, Schmeidler J, Beecham G, et al. FE65 binds Teashirt, inhibiting expression of the primate-specific caspase-4. PLoS ONE. 2009;4:e5071 pubmed publisher
Ashe A, Morgan D, Whitelaw N, Bruxner T, Vickaryous N, Cox L, et al. A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development. Genome Biol. 2008;9:R182 pubmed publisher
Stolt C, Lommes P, Hillgärtner S, Wegner M. The transcription factor Sox5 modulates Sox10 function during melanocyte development. Nucleic Acids Res. 2008;36:5427-40 pubmed publisher
Iwamoto F, Stadler M, Chalupníková K, Oakeley E, Nagamine Y. Transcription-dependent nucleolar cap localization and possible nuclear function of DExH RNA helicase RHAU. Exp Cell Res. 2008;314:1378-91 pubmed publisher
Le May N, Mansuroglu Z, Léger P, Josse T, Blot G, Billecocq A, et al. A SAP30 complex inhibits IFN-beta expression in Rift Valley fever virus infected cells. PLoS Pathog. 2008;4:e13 pubmed publisher
Diaz Martinez L, Giménez Abián J, Clarke D. Regulation of centromeric cohesion by sororin independently of the APC/C. Cell Cycle. 2007;6:714-24 pubmed
Simonsson M, Heldin C, Ericsson J, Grönroos E. The balance between acetylation and deacetylation controls Smad7 stability. J Biol Chem. 2005;280:21797-803 pubmed
product information
Catalog Number :
05-100
Subcategory :
Cell Signaling
Product Name :
Anti-HDAC1 Antibody, clone 2E10
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q13547
Host Name :
Mouse
Antigen :
HDAC1
Clone :
2E10
Conjugate :
Culture Supernatant
Isotype :
IgG1
Product Description :
Anti-HDAC1 Antibody, clone 2E10
Cross Reactivity :
Human;Mouse
Immunogen :
KLH-conjugated, synthetic peptide (C-EEKPEAKGVKEEVKLA) corresponding to amino acids 467-482 of mouse HDAC1. The immunizing sequence is identical in human.
Specificity :
HDAC-1
Package Size :
100 μL
Uses :
Immunoprecipitation;Western Blotting
Storage :
2 years at -20°C from date of shipment.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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