phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

catalog :

04-953

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

A5S

reactivity :

human, mouse, rat, dogs, zebrafish

application :

western blot, immunohistochemistry - paraffin section

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 1f	MacFarlane E, Parker S, Shin J, Kang B, Ziegler S, Creamer T, et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest. 2019;129:659-675 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 4a	Jiang L, Han X, Wang J, Wang C, Sun X, Xie J, et al. SHP-1 regulates hematopoietic stem cell quiescence by coordinating TGF-β signaling. J Exp Med. 2018;215:1337-1347 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 4m	Meester J, Vandeweyer G, Pintelon I, Lammens M, Van Hoorick L, De Belder S, et al. Loss-of-function mutations in the X-linked biglycan gene cause a severe syndromic form of thoracic aortic aneurysms and dissections. Genet Med. 2017;19:386-395 pubmed publisher
western blot; rat; 1:2500; loading ...; fig 1a	Djamali A, Wilson N, Sadowski E, Zha W, Niles D, Hafez O, et al. Nox2 and Cyclosporine-Induced Renal Hypoxia. Transplantation. 2016;100:1198-210 pubmed publisher
western blot; mouse; 1:1000; fig 6	Sawada S, Chosa N, Takizawa N, Yokota J, Igarashi Y, Tomoda K, et al. Establishment of mesenchymal stem cell lines derived from the bone marrow of green fluorescent protein-transgenic mice exhibiting a diversity in intracellular transforming growth factor-Î² and bone morphogenetic protein signaling. Mol Med Rep. 2016;13:2023-31 pubmed publisher
western blot; human; 1:500; fig 5	Aspalter I, Gordon E, Dubrac A, Ragab A, Narloch J, Vizan P, et al. Alk1 and Alk5 inhibition by Nrp1 controls vascular sprouting downstream of Notch. Nat Commun. 2015;6:7264 pubmed publisher
western blot; human western blot; rat western blot; mouse; fig 5	Juin A, Planus E, Guillemot F, Horáková P, Albiges Rizo C, Genot E, et al. Extracellular matrix rigidity controls podosome induction in microvascular endothelial cells. Biol Cell. 2013;105:46-57 pubmed publisher
	Kirmizitas A, Meiklejohn S, Ciau Uitz A, Stephenson R, Patient R. Dissecting BMP signaling input into the gene regulatory networks driving specification of the blood stem cell lineage. Proc Natl Acad Sci U S A. 2017;114:5814-5821 pubmed publisher
	Portela R, Fadl Alla B, Pondenis H, Byrum M, Garrett L, Wycislo K, et al. Pro-tumorigenic effects of transforming growth factor beta 1 in canine osteosarcoma. J Vet Intern Med. 2014;28:894-904 pubmed publisher
	Zhang S, Li J, Lea R, Amaya E, Dorey K. A functional genome-wide in vivo screen identifies new regulators of signalling pathways during early Xenopus embryogenesis. PLoS ONE. 2013;8:e79469 pubmed publisher
	Ramel M, Hill C. The ventral to dorsal BMP activity gradient in the early zebrafish embryo is determined by graded expression of BMP ligands. Dev Biol. 2013;378:170-82 pubmed publisher
	Nagasawa A, Yoshimura K, Suzuki R, Mikamo A, Yamashita O, Ikeda Y, et al. Important role of the angiotensin II pathway in producing matrix metalloproteinase-9 in human thoracic aortic aneurysms. J Surg Res. 2013;183:472-7 pubmed publisher
	Schmit J, Pondenis H, Barger A, Borst L, Garrett L, Wypij J, et al. Cathepsin K expression and activity in canine osteosarcoma. J Vet Intern Med. 2012;26:126-34 pubmed publisher
	Moyano J, Greciano P, Buschmann M, Koch M, Matlin K. Autocrine transforming growth factor-{beta}1 activation mediated by integrin {alpha}V{beta}3 regulates transcriptional expression of laminin-332 in Madin-Darby canine kidney epithelial cells. Mol Biol Cell. 2010;21:3654-68 pubmed publisher

product information

Catalog Number :

04-953

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q15796

Host Name :

Rabbit

Antigen :

phospho-Smad2 (Ser465/467)

Clone :

A5S

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

Cross Reactivity :

Canine;Human;Mouse;Zebrafish

Background :

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Immunogen :

KLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2

Specificity :

Recognizes phosphorylated Smad2. No Cross-reactivity with Smad3.

Package Size :

100 μL

Uses :

Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.