Smad2/3 Antibody, clone C4T, rabbit monoclonal | EMD Millipore 04-914 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Smad2/3 Antibody, clone C4T, rabbit monoclonal

catalog :

04-914

quantity :

100 μl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

C4T

reactivity :

human, mouse

application :

western blot

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 3b	Ying W, Li X, Rangarajan S, Feng W, Curtis L, Sanders P. Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury. J Clin Invest. 2019;129:2792-2806 pubmed publisher

product information

Catalog Number :

04-914

Subcategory :

Cell Signaling

Product Name :

Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q13485

Host Name :

Rabbit

Antigen :

Smad2/3

Clone :

C4T

Product Description :

Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. Smad 4 mutations are frequently found in cancer, all of which cluster to the MH1 and MH2 domains of the protein. Those in the MH2 domain affect the ability of the protein to homotrimerize. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

ALT Names :

Deletion target in pancreatic carcinoma 4;MAD, mothers against decapentaplegic homolog 4;MAD, mothers against decapentaplegic homolog 4 (Drosophila) ;Mothers against DPP homolog 4;SMAD 4;SMAD family member 4;SMAD, mothers against DPP homolog 4;SMAD, mothers against DPP homolog 4 (Drosophila);deleted in pancreatic carcinoma locus 4 ;mothers against decapentaplegic homolog 4;mothers against decapentaplegic, Drosophila, homolog of, 4

Immunogen :

Fusion protein corresponding to amino acids 186-273 of human Smad2.

Specificity :

Recognizes Smad2, Mr 55-60 kDa and Smad3, Mr 50 kDa.

Package Size :

100 μl

Uses :

Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.