Src Antibody, clone N6L, rabbit monoclonal | EMD Millipore 04-889 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Src Antibody, clone N6L, rabbit monoclonal

catalog :

04-889

quantity :

100 μl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

N6L

reactivity :

human

application :

western blot

product information

Catalog Number :

04-889

Subcategory :

Cell Structure

Product Name :

Anti-Src Antibody, clone N6L, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12931

Host Name :

Rabbit

Antigen :

Src

Clone :

N6L

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-Src Antibody, clone N6L, rabbit monoclonal

Cross Reactivity :

Human

Background :

pp60c-Src is the prototype non-receptor tyrosine kinase. Homology to Src in three regions is shared by a number of proteins: SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain. The Src SH2 domain binds to phospho-Tyr527 (phosphorylated by Csk) within the Src molecule, causing Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype

ALT Names :

proto-oncogene tyrosine-protein kinase SRC;protooncogene SRC, Rous sarcoma;tyrosine kinase pp60c-src;tyrosine-protein kinase SRC-1;v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog;v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Immunogen :

N-terminal 6His-tagged recombinant full-length human Src expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specificity :

Recognizes Src. Crossreacts with Yes and weakly with Fyn. No crossreactivity observed with Lck or Lyn.

Package Size :

100 μl

Uses :

Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.