ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal | EMD Millipore 04-841 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

catalog :

04-841

quantity :

200 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

A9W4

reactivity :

hamsters, human, mouse, rat, bovine

application :

western blot, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig s2e	Jerić I, Maurer G, Cavallo A, Raguz J, Desideri E, Tarkowski B, et al. A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis. Nat Commun. 2016;7:13781 pubmed publisher
western blot; hamsters; 1:500; fig 2	Newell Litwa K, Badoual M, Asmussen H, Patel H, Whitmore L, Horwitz A. ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity. J Cell Biol. 2015;210:225-42 pubmed publisher

product information

Catalog Number :

04-841

Subcategory :

Cell Signaling

Product Name :

Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q62868

Host Name :

Rabbit

Antigen :

ROKα/ROCK-II

Clone :

A9W4

Conjugate :

Culture Supernatant

Product Description :

Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat;Bovine

Background :

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

ALT Names :

RhoA-binding kinase 2;Rho-associated, coiled-coil containing protein kinase 2;Rho-associated, coiled-coil-containing protein kinase II;p150 ROK-alpha;p164 ROCK-2;ROCK-II;ROKalpha;Rock2

Immunogen :

6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.

Specificity :

Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.

Package Size :

200 μL

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.