dimethyl-Histone H3 (Lys79) Antibody, clone NL59, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

dimethyl-Histone H3 (Lys79) Antibody, clone NL59, rabbit monoclonal

catalog :

04-835

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

NL59

reactivity :

human, mouse, brewer's yeast

application :

western blot, chromatin immunoprecipitation

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; brewer's yeast; 1:2000-1:3000; loading ...; fig 1a	Young C, Hillyer C, Hokamp K, Fitzpatrick D, Konstantinov N, Welty J, et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics. 2017;18:107 pubmed publisher
chromatin immunoprecipitation; mouse	Weishaupt H, Attema J. A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip. Biol Proced Online. 2010;12:1-17 pubmed publisher
	van Welsem T, Korthout T, Ekkebus R, Morais D, Molenaar T, van Harten K, et al. Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism. Nucleic Acids Res. 2018;46:11251-11261 pubmed publisher
	Malmgren S, Spégel P, Danielsson A, Nagorny C, Andersson L, Nitert M, et al. Coordinate changes in histone modifications, mRNA levels, and metabolite profiles in clonal INS-1 832/13 β-cells accompany functional adaptations to lipotoxicity. J Biol Chem. 2013;288:11973-87 pubmed publisher
	Caretti A, Sirchia S, Tabano S, Zulueta A, Dall Olio F, Trinchera M. DNA methylation and histone modifications modulate the ?1,3 galactosyltransferase ?3Gal-T5 native promoter in cancer cells. Int J Biochem Cell Biol. 2012;44:84-90 pubmed publisher
	M ller Taubenberger A, B nisch C, F rbringer M, Wittek F, Hake S. The histone methyltransferase Dot1 is required for DNA damage repair and proper development in Dictyostelium. Biochem Biophys Res Commun. 2011;404:1016-22 pubmed publisher
	Psathas J, Zheng S, Tan S, Reese J. Set2-dependent K36 methylation is regulated by novel intratail interactions within H3. Mol Cell Biol. 2009;29:6413-26 pubmed publisher

product information

Catalog Number :

04-835

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Rabbit

Antigen :

dimethyl-Histone H3 (Lys79)

Clone :

NL59

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, rabbit monoclonal

Cross Reactivity :

Human;Vertebrates

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Dimethylation of Lys79 on histone H3 is associated with active gene transcription and is a marker for euchromatin. Histone H3 is methylated on Lys79 by the histone methyltransferase Dot1L.

ALT Names :

H3K79me2;Histone H3 (di methyl K79)

Immunogen :

KLH-conjugated synthetic peptide containing the sequence DF me2 KTD corresponding to dimethyl-lysine at position 79 of histone H3. The immunizing sequence is identical in human, mouse, rat and yeast. A C-terminal cysteine was added to facilitate conjugation.

Specificity :

Recognizes dimethyl-histone H3 (Lys79).

Package Size :

100 μL

Uses :

Western Blotting;Dot Blot;Multiplexing;ChIP-seq;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.