phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

catalog :

04-817

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

MC463

reactivity :

freshwater planarian, Saccharomyces cerevisiae S288C, human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; freshwater planarian; 1:25; fig 2g	Deochand M, Birkholz T, Beane W. Temporal regulation of planarian eye regeneration. Regeneration (Oxf). 2016;3:209-221 pubmed
chromatin immunoprecipitation; Saccharomyces cerevisiae S288C	Weiner A, Chen H, Liu C, Rahat A, Klien A, Soares L, et al. Systematic dissection of roles for chromatin regulators in a yeast stress response. PLoS Biol. 2012;10:e1001369 pubmed publisher
western blot; mouse western blot; human	Verma R, Xu X, Jaiswal M, Olsen C, Mears D, Caretti G, et al. In vitro profiling of epigenetic modifications underlying heavy metal toxicity of tungsten-alloy and its components. Toxicol Appl Pharmacol. 2011;253:178-87 pubmed publisher
chromatin immunoprecipitation; human western blot; human; 1:2000	Egelhofer T, Minoda A, Klugman S, Lee K, Kolasinska Zwierz P, Alekseyenko A, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18:91-3 pubmed publisher
	Waraky A, Akopyan K, Parrow V, StrÃ¶mberg T, Axelson M, AbrahmsÃ©n L, et al. Picropodophyllin causes mitotic arrest and catastrophe by depolymerizing microtubules via insulin-like growth factor-1 receptor-independent mechanism. Oncotarget. 2014;5:8379-92 pubmed
	Campbell A, Hsiung C, Blobel G. Comparative analysis of mitosis-specific antibodies for bulk purification of mitotic populations by fluorescence-activated cell sorting. Biotechniques. 2014;56:90-94 pubmed publisher
	Burke S, Updegraff B, Bellich R, Goff M, Lu D, Minkin S, et al. Regulation of iNOS gene transcription by IL-1? and IFN-? requires a coactivator exchange mechanism. Mol Endocrinol. 2013;27:1724-42 pubmed publisher
	Luo L, Chen S, Gong Q, Luo N, Lei Y, Guo J, et al. Hepatitis B virus X protein modulates remodelling of minichromosomes related to hepatitis B virus replication in HepG2 cells. Int J Mol Med. 2013;31:197-204 pubmed publisher
	Zhu S, Pearson B. The Retinoblastoma pathway regulates stem cell proliferation in freshwater planarians. Dev Biol. 2013;373:442-52 pubmed publisher
	Vasilyev A, Liu Y, Hellman N, Pathak N, Drummond I. Mechanical stretch and PI3K signaling link cell migration and proliferation to coordinate epithelial tubule morphogenesis in the zebrafish pronephros. PLoS ONE. 2012;7:e39992 pubmed publisher
	Hamon M, Cossart P. K+ efflux is required for histone H3 dephosphorylation by Listeria monocytogenes listeriolysin O and other pore-forming toxins. Infect Immun. 2011;79:2839-46 pubmed publisher
	Fraguas S, Barberán S, Cebria F. EGFR signaling regulates cell proliferation, differentiation and morphogenesis during planarian regeneration and homeostasis. Dev Biol. 2011;354:87-101 pubmed publisher
	Gong Q, Chen S, Guo J, Sun H, Zheng G, Liu Q, et al. Chromosome remodeling related to hepatitis B virus replication in HepG2 cells. DNA Cell Biol. 2011;30:347-54 pubmed publisher
	Ahangarani R, Janssens W, Carlier V, VanderElst L, VandenDriessche T, Chuah M, et al. Retroviral vectors induce epigenetic chromatin modifications and IL-10 production in transduced B cells via activation of toll-like receptor 2. Mol Ther. 2011;19:711-22 pubmed publisher
	Dellaire G, Ching R, Ahmed K, Jalali F, Tse K, Bristow R, et al. Promyelocytic leukemia nuclear bodies behave as DNA damage sensors whose response to DNA double-strand breaks is regulated by NBS1 and the kinases ATM, Chk2, and ATR. J Cell Biol. 2006;175:55-66 pubmed

product information

Catalog Number :

04-817

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

phospho-Histone H3 (Ser10)

Clone :

MC463

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

Cross Reactivity :

Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine

ALT Names :

H3S10P;Histone H3 (phospho S10)

Immunogen :

Peptide containing the sequence RK[pS]TG. in which pS corresponds to phospho-serine at position 10 of human histone H3

Specificity :

Histone H3 phosphorylated at Serine 10

Package Size :

100 µL

Uses :

Immunofluorescence;Western Blotting;Immunocytochemistry;ChIP-seq;Dot Blot;Multiplexing;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.