trimethyl(Lys9)-phospho(Ser10)-Histone H3 Antibody, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

trimethyl(Lys9)-phospho(Ser10)-Histone H3 Antibody, rabbit monoclonal

catalog :

04-809

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

Catalog Number :

04-809

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-trimethyl(Lys9)-phospho(Ser10)-Histone H3 Antibody, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

trimethyl(Lys9)-phospho(Ser10)-Histone H3

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-trimethyl(Lys9)-phospho(Ser10)-Histone H3 Antibody, rabbit monoclonal

Cross Reactivity :

Human

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely via acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α/β. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation.

ALT Names :

H3K9me3S10P;Histone H3 (tri methyl K9, phospho S10)

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence …ARme3KpST… in which me3K corresponds to trimethyl-lysine 9 and pS to phosphoserine 10 of human histone H3.

Specificity :

Recognizes histone H3 containing the dual modifications of trimethylation of Lys9 and phosphorylation of Ser10. Does not detect single modifications or other dual modifications of histone H3.

Package Size :

100 µL

Uses :

Western Blotting;Peptide Inhibition Assay;Dot Blot;Multiplexing

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.