dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal

catalog :

04-808

quantity :

200 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

20.2

reactivity :

human

application :

western blot, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; fig 1	Stein C, Nötzold R, Riedl S, Bouchard C, Bauer U. The Arginine Methyltransferase PRMT6 Cooperates with Polycomb Proteins in Regulating HOXA Gene Expression. PLoS ONE. 2016;11:e0148892 pubmed publisher
western blot; human; 1:2000	Egelhofer T, Minoda A, Klugman S, Lee K, Kolasinska Zwierz P, Alekseyenko A, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18:91-3 pubmed publisher
	Dhawan S, Dirice E, Kulkarni R, Bhushan A. Inhibition of TGF-β Signaling Promotes Human Pancreatic β-Cell Replication. Diabetes. 2016;65:1208-18 pubmed publisher
	Slade D, Subramanian V, Fuhrmann J, Thompson P. Chemical and biological methods to detect post-translational modifications of arginine. Biopolymers. 2014;101:133-43 pubmed publisher

product information

Catalog Number :

04-808

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

dimethyl-Histone H3 (Arg2)

Clone :

20.2

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal

Cross Reactivity :

Human;Vertebrates

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone H3 Arg2 methylation is a widespread silencing modification that inhibits histone H3 Lys4 trimethylation.

ALT Names :

H3R2me2;Histone H3 (di methyl R2)

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence …Ame2RTKQ…, in which me2R corresponds to dimethyl-arginine 2 of human histone H3.

Specificity :

Recognizes dimethyl-arginine at residue 2 of histone H3, Mr 17kDa. Additional unidentified bands above 50 kDa are detected in some samples.

Package Size :

200 µL

Uses :

Dot Blot;Multiplexing;Peptide Inhibition Assay;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.