phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

catalog :

04-802

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

NL50

reactivity :

human, mouse, rat, chicken, bovine

application :

western blot

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; loading ...; fig 7a	Shu M, Hu X, Hung Z, Huang D, Zhang S. Effects of tanshinone IIA on fibrosis in a rat model of cirrhosis through heme oxygenase-1, inflammation, oxidative stress and apoptosis. Mol Med Rep. 2016;13:3036-42 pubmed publisher

product information

Catalog Number :

04-802

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31749

Host Name :

Rabbit

Antigen :

phospho-Akt1/PKBα (Thr308)

Clone :

NL50

Isotype :

IgG

Product Description :

Anti-phospho-Akt1/PKBα (Thr308) Antibody, clone NL50, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Bovine;Chicken;Rat

Background :

Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3, which is inactivated by this phosphorylation, the FoxO family of transcription factors, PRAS40, AS160.

ALT Names :

Protein kinase B;RAC-alpha serine/threonine-protein kinase ;murine thymoma viral (v-akt) oncogene homolog-1;rac protein kinase alpha ;v-akt murine thymoma viral oncogene homolog 1

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence . MK[pT]FC. in which pT corresponds to phospho-threonine at residue 308 of mouse Akt. The Akt1 sequence is identical to human, rat, bovine and chicken Akt1. Human and mouse Akt2 shares 11/12 amino acids and rat Akt3 shares 10/12 amino acids with the peptide immunogen sequence.

Specificity :

Recognizes phosphorylated Akt1/PKBα, Mr 60 kDa. Cross-reactivity observed with Akt2, but not with Akt3.

Package Size :

100 μL

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.