Akt1/PKBα Antibody, clone AW24, rabbit monoclonal | EMD Millipore 04-796 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Akt1/PKBα Antibody, clone AW24, rabbit monoclonal

catalog :

04-796

quantity :

100 μl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AW24

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; human; tbl s3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
	González Hedström D, Amor S, de la Fuente Fernández M, Tejera Muñoz A, Priego T, Martín A, et al. A Mixture of Algae and Extra Virgin Olive Oils Attenuates the Cardiometabolic Alterations Associated with Aging in Male Wistar Rats. Antioxidants (Basel). 2020;9: pubmed publisher
	de la Fuente Fernández M, González Hedström D, Amor S, Tejera Muñoz A, Fernandez N, Monge L, et al. Supplementation with a Carob (Ceratonia siliqua L.) Fruit Extract Attenuates the Cardiometabolic Alterations Associated with Metabolic Syndrome in Mice. Antioxidants (Basel). 2020;9: pubmed publisher
	Iacovides D, Johnson A, Wang N, Boddapati S, Korkola J, Gray J. Identification and quantification of AKT isoforms and phosphoforms in breast cancer using a novel nanofluidic immunoassay. Mol Cell Proteomics. 2013;12:3210-20 pubmed publisher
	Moore S, van den Bosch M, Hunter R, Sakamoto K, Poole A, Hers I. Dual regulation of glycogen synthase kinase 3 (GSK3)?/? by protein kinase C (PKC)? and Akt promotes thrombin-mediated integrin ?IIb?3 activation and granule secretion in platelets. J Biol Chem. 2013;288:3918-28 pubmed publisher
	Lu X, Tompkins R, Fischman A. SILAM for quantitative proteomics of liver Akt1/PKB? after burn injury. Int J Mol Med. 2012;29:461-71 pubmed publisher
	Siepmann M, Kumar S, Mayer G, Walter J. Casein kinase 2 dependent phosphorylation of neprilysin regulates receptor tyrosine kinase signaling to Akt. PLoS ONE. 2010;5: pubmed publisher
	Keeshan K, Bailis W, Dedhia P, Vega M, Shestova O, Xu L, et al. Transformation by Tribbles homolog 2 (Trib2) requires both the Trib2 kinase domain and COP1 binding. Blood. 2010;116:4948-57 pubmed publisher

product information

Catalog Number :

04-796

Subcategory :

Cell Signaling

Product Name :

Anti-Akt1/PKBα Antibody, clone AW24, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31749

Host Name :

Rabbit

Antigen :

Akt1/PKBα

Clone :

AW24

Isotype :

IgG

Product Description :

Anti-Akt1/PKBα Antibody, clone AW24, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

ALT Names :

AKT1 kinase;Protein kinase B;RAC-alpha serine/threonine-protein kinase;rac protein kinase alpha;v-akt murine thymoma viral oncogene homolog 1

Immunogen :

Sythetic peptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1/PKBα.

Specificity :

This antibody recognizes Akt1/PKBα.

Package Size :

100 μl

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.