mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

catalog :

04-791

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AW304

reactivity :

human

application :

western blot, chromatin immunoprecipitation

citations: 5

Reference
Kougioumtzi A, Tsaparas P, Magklara A. Deep sequencing reveals new aspects of progesterone receptor signaling in breast cancer cells. PLoS ONE. 2014;9:e98404 pubmed publisher
Satoh T, Tajima M, Wakita D, Kitamura H, Nishimura T. The development of IL-17/IFN-?-double producing CTLs from Tc17 cells is driven by epigenetic suppression of Socs3 gene promoter. Eur J Immunol. 2012;42:2329-42 pubmed publisher
Larsson L, Thorbert Mros S, Rymo L, Berglundh T. Influence of epigenetic modifications of the interleukin-10 promoter on IL10 gene expression. Eur J Oral Sci. 2012;120:14-20 pubmed publisher
Franklin S, Chen H, Mitchell Jordan S, Ren S, Wang Y, Vondriska T. Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth. Mol Cell Proteomics. 2012;11:M111.014258 pubmed publisher
Zager R, Johnson A. Progressive histone alterations and proinflammatory gene activation: consequences of heme protein/iron-mediated proximal tubule injury. Am J Physiol Renal Physiol. 2010;298:F827-37 pubmed publisher

product information

Catalog Number :

04-791

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

mono/di/trimethyl-Histone H3 (Lys4)

Clone :

AW304

Isotype :

IgG

Product Description :

Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

Cross Reactivity :

Human;Vertebrates

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone methylation is primarily associated with heterochromatin and gene silencing.

ALT Names :

H3K4me;Histone H3 (methyl K4)

Immunogen :

KLH-conjugated, synthetic peptide containing …Tme2KQT… in which me2K corresponds to dimethyl-lysine at residue 4 of human histone H3.

Specificity :

Monomethyl, dimethyl, and trimethyl histone H3 (Lys4). Additional unidentified bands above 50 kDa are detected in some samples

Package Size :

100 μL

Uses :

Western Blotting;Peptide Inhibition Assay;Multiplexing;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.